Chemical tags facilitate the sensing of individual DNA strands with nanopores

被引：49

作者：

Mitchell, Nick ^{[1
]}

Howorka, Stefan ^{[1
]}

机构：

[1] UCL, Dept Chem, London WC1H 0AJ, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 30期

基金：

英国工程与自然科学研究理事会;

关键词：

biosensors; DNA; nanopores; peptides; single-molecule studies;

D O I：

10.1002/anie.200800183

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) The base resolution in the detection of single DNA strands with nanopores can be improved by attaching chemical tags to individual bases. Different chemical tags give rise to characteristic current signatures when a DNA molecule passes through a nanopore (see picture). This sequence-specific sensing with modified DNA is independent of pore engineering and can potentially be applied to analyze nucleic acids with a wide range of solid-state nanopores. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：5565 / 5568

页数：4

相关论文

共 23 条

[1] Recognizing a single base in an individual DNA strand:: A step toward DNA sequencing in nanopores [J].

Ashkenasy, N ;

Sánchez-Quesada, J ;

Bayley, H ;

Ghadiri, MR .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (09) :1401-1404

[2]

Ashkenasy N., 2005, Angewandte Chemie, V117, P1425

[3] Toward single molecule DNA sequencing:: Direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter [J].

Astier, Y ;

Braha, O ;

Bayley, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (05) :1705-1710

[4] Sequencing single molecules of DNA [J].

Bayley, Hagan .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2006, 10 (06) :628-637

[5] DNA sequencing: generation next-next [J].

Blow, Nathan .

NATURE METHODS, 2008, 5 (03) :267-+

[6] Determination of RNA orientation during translocation through a biological nanopore [J].

Butler, TZ ;

Gundlach, JH ;

Troll, MA .

BIOPHYSICAL JOURNAL, 2006, 90 (01) :190-199

[7] Solid-state nanopores [J].

Dekker, Cees .

NATURE NANOTECHNOLOGY, 2007, 2 (04) :209-215

[8] Recapturing and trapping single molecules with a solid-state nanopore [J].

Gershow, Marc ;

Golovchenko, J. A. .

NATURE NANOTECHNOLOGY, 2007, 2 (12) :775-779

[9] Sequence-specific detection of individual DNA strands using engineered nanopores [J].

Howorka, S ;

Cheley, S ;

Bayley, H .

NATURE BIOTECHNOLOGY, 2001, 19 (07) :636-639

[10] Characterization of individual polynucleotide molecules using a membrane channel [J].

Kasianowicz, JJ ;

Brandin, E ;

Branton, D ;

Deamer, DW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) :13770-13773