Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification

被引:68
作者
Budin, Ghyslain [1 ]
Yang, Katherine S. [1 ]
Reiner, Thomas [1 ]
Weissleder, Ralph [1 ,2 ]
机构
[1] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA
[2] Harvard Univ, Sch Med, Boston, MA 02115 USA
基金
美国国家卫生研究院;
关键词
cancer; cell cycle; cycloaddition; live cell imaging; polo-like kinase; BOX DOMAIN; PLK1; SUBSTRATE; PROPHASE; PHOSPHORYLATION; DIVISION; CDC25C; CELLS; TEAM;
D O I
10.1002/anie.201103273
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:9378 / 9381
页数:4
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