Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification

被引：68

作者：

Budin, Ghyslain ^{[1
]}

Yang, Katherine S. ^{[1
]}

Reiner, Thomas ^{[1
]}

Weissleder, Ralph ^{[1
,2
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Boston, MA 02115 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 40期

基金：

美国国家卫生研究院;

关键词：

cancer; cell cycle; cycloaddition; live cell imaging; polo-like kinase; BOX DOMAIN; PLK1; SUBSTRATE; PROPHASE; PHOSPHORYLATION; DIVISION; CDC25C; CELLS; TEAM;

D O I：

10.1002/anie.201103273

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9378 / 9381

页数：4

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