Downstream processing of virus-like particles with aqueous two-phase systems: Applications and challenges

被引：12
作者：
Leong, Hui Yi [1 ]
Fu, Xiao-Qian [1 ]
Show, Pau Loke [2 ]
Yao, Shan-Jing [1 ]
Lin, Dong-Qiang [1 ]
机构：
[1] Zhejiang Univ, Key Lab Biomass Chem Engn, Minist Educ, Zhejiang Key Lab Smart Biomat,Coll Chem & Biol En, Hangzhou 310027, Peoples R China
[2] Univ Nottingham Malaysia, Fac Sci & Engn, Dept Chem & Environm Engn, Broga Rd, Semenyih 43500, Selangor Darul, Malaysia
来源：
JOURNAL OF SEPARATION SCIENCE | 2022年 / 45卷 / 12期
基金：
中国国家自然科学基金;
关键词：
aqueous two-phase systems; continuous bio-manufacturing; extraction; integrated processes; virus-like particles; BETACYANINS EXTRACTION; MONOCLONAL-ANTIBODIES; HYLOCEREUS-POLYRHIZUS; PURIFICATION; VACCINES; FLOTATION; RECOVERY; POLYMER; TRENDS; FLESH;
D O I：
10.1002/jssc.202100947
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The advancement of recombinant virus-like particle-based vaccines has attracted global attention owing to substantially safety and high efficacy in provoking a protective immunity against various chronic and infectious diseases in humans and animals. A robust, low-cost, and scalability separation and purification technology is of utmost importance in the downstream processing of recombinant virus-like particles to produce affordable and safe vaccines. Being a relatively simple, environmentally friendly, and efficient biomolecules recovery approach, aqueous two-phase systems have received great attention from researchers worldwide. This review aims to highlight the challenges and outlook in addition to the current applications of aqueous two-phase systems in downstream processing of virus-like particles. The efforts will confidently reinforce scholars' knowledge and fill in the valuable research gap in the aspect of concerning recombinant virus-like particle-based vaccines development, particularly related to the virus-like particles downstream production processes.
引用
收藏
页码：2064 / 2076
页数：14
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