Downstream processing of virus-like particles with aqueous two-phase systems: Applications and challenges

被引：12
作者：
Leong, Hui Yi [1 ]
Fu, Xiao-Qian [1 ]
Show, Pau Loke [2 ]
Yao, Shan-Jing [1 ]
Lin, Dong-Qiang [1 ]
机构：
[1] Zhejiang Univ, Key Lab Biomass Chem Engn, Minist Educ, Zhejiang Key Lab Smart Biomat,Coll Chem & Biol En, Hangzhou 310027, Peoples R China
[2] Univ Nottingham Malaysia, Fac Sci & Engn, Dept Chem & Environm Engn, Broga Rd, Semenyih 43500, Selangor Darul, Malaysia
来源：
JOURNAL OF SEPARATION SCIENCE | 2022年 / 45卷 / 12期
基金：
中国国家自然科学基金;
关键词：
aqueous two-phase systems; continuous bio-manufacturing; extraction; integrated processes; virus-like particles; BETACYANINS EXTRACTION; MONOCLONAL-ANTIBODIES; HYLOCEREUS-POLYRHIZUS; PURIFICATION; VACCINES; FLOTATION; RECOVERY; POLYMER; TRENDS; FLESH;
D O I：
10.1002/jssc.202100947
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The advancement of recombinant virus-like particle-based vaccines has attracted global attention owing to substantially safety and high efficacy in provoking a protective immunity against various chronic and infectious diseases in humans and animals. A robust, low-cost, and scalability separation and purification technology is of utmost importance in the downstream processing of recombinant virus-like particles to produce affordable and safe vaccines. Being a relatively simple, environmentally friendly, and efficient biomolecules recovery approach, aqueous two-phase systems have received great attention from researchers worldwide. This review aims to highlight the challenges and outlook in addition to the current applications of aqueous two-phase systems in downstream processing of virus-like particles. The efforts will confidently reinforce scholars' knowledge and fill in the valuable research gap in the aspect of concerning recombinant virus-like particle-based vaccines development, particularly related to the virus-like particles downstream production processes.
引用
收藏
页码：2064 / 2076
页数：14
相关论文
共 71 条
[1]   Rotavirus-like particles primary recovery from insect cells in aqueous two-phase systems [J].
Benavides, Jorge ;
Mena, Jimmy A. ;
Cisneros-Ruiz, Mayra ;
Ramirez, Octavio T. ;
Palomares, Laura A. ;
Rito-Palomares, Marco .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 842 (01) :48-57
[2]   Emerging aqueous two-phase systems: from fundamentals of interfaces to biomedical applications [J].
Chao, Youchuang ;
Shum, Ho Cheung .
CHEMICAL SOCIETY REVIEWS, 2020, 49 (01) :114-142
[3]   Purification of virus-like particles of recombinant human papillomavirus type 11 major capsid protein L1 from Saccharomyces cerevisiae [J].
Cook, JC ;
Joyce, JG ;
George, HA ;
Schultz, LD ;
Hurni, WM ;
Jansen, KU ;
Hepler, RW ;
Ip, C ;
Lowe, RS ;
Keller, PM ;
Lehman, ED .
PROTEIN EXPRESSION AND PURIFICATION, 1999, 17 (03) :477-484
[4]   Partitioning of proteins using a hydrophobically modified ethylene oxide/SDS aqueous two-phase system [J].
Deng, G ;
Yao, SJ ;
Lin, DQ .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2005, 21 (6-7) :1209-1214
[5]   Aqueous micellar two-phase system composed of hyamine-type hydrophobically modified ethylene oxide and application for cytochrome P450BM-3 separation [J].
Deng, Gang ;
Lin, Dong-Qiang ;
Yao, Shan-Jing ;
Mei, Le-He .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 852 (1-2) :167-173
[6]   HPV vaccines to prevent cervical cancer and genital warts: an update [J].
Dochez, Carine ;
Bogers, Johannes J. ;
Verhelst, Rita ;
Rees, Helen .
VACCINE, 2014, 32 (14) :1595-1601
[7]   Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems [J].
Dong, Beitao ;
Yuan, Xiaofan ;
Zhao, Qingsheng ;
Feng, Qingming ;
Liu, Boyan ;
Guo, Yuanheng ;
Zhao, Bing .
JOURNAL OF SEPARATION SCIENCE, 2015, 38 (07) :1194-1203
[8]   Next generation vaccines and vectors: Designing downstream processes for recombinant protein-based virus-like particles [J].
Effio, Christopher Ladd ;
Hubbuch, Juergen .
BIOTECHNOLOGY JOURNAL, 2015, 10 (05) :715-U100
[9]   Downstream processing of virus-like particles: Single-stage and multi-stage aqueous two-phase extraction [J].
Effio, Christopher Ladd ;
Wenger, Lukas ;
Oetes, Ozan ;
Oelmeier, Stefan A. ;
Kneusel, Richard ;
Hubbuch, Juergen .
JOURNAL OF CHROMATOGRAPHY A, 2015, 1383 :35-46
[10]   Continuous aqueous two-phase extraction: From microfluidics to integrated biomanufacturing [J].
Ferreira-Faria, Diogo ;
Aires-Barros, M. Raquel ;
Azevedo, Ana M. .
FLUID PHASE EQUILIBRIA, 2020, 508
12345678