Successful Cryopreservation of Human Embryonic Stem Cell-Derived Cardiomyocytes Using Vitirification

被引：0
作者：
Kim, Yoon Young [1 ]
Ku, Seung-Yup [1 ,2 ]
Oh, Sun Kyung [1 ,2 ]
Kim, Seok Hyun [1 ,2 ]
Moon, Shin Yong [1 ,2 ]
Choi, Young Min [1 ,2 ]
机构：
[1] Med Res Ctr, Inst Reprod Med & Populat, Seoul, South Korea
[2] Seoul Natl Univ, Coll Med, Dept Obstet & Gynecol, Seoul 110744, South Korea
来源：
TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2011年 / 8卷 / 01期
关键词：
hESC-derived CMs; cryopreservation; vitrification; VITRIFICATION; SURVIVAL; DIFFERENTIATION; EFFICIENT; VIABILITY;
D O I：
暂无
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Human embryonic stem cell (hESC)-derived, differentiated cells are an alternative cell source for many intractable diseases in regenerative medicine. However, the proper environments for long-term storage of hESC-derived cells are still unknown. In this study, we compared the recovery rate of two different cryopreservation methods: mass cryopreservation vs. vitrification. hESC-derived cardiomyocytes (CMs) at two stages, day 13 (pre-contraction) and day 16 (post-contraction), were frozen in the presence of ROCK inhibitors. Thawed-CMs of day 13 from both groups showed higher survival rates than those of day 16. Thawed cells retained the characteristics of cardiomyocytes after long-term storage. Contraction of thawed CMs appeared earlier ill vitrification group than in mass cryopreserved group. This study demonstrated that vitrification can be a useful method for the long-term storage of hESC-derived CMs.
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页码：55 / 61
页数：7
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