Vitamin D analog EB1089 could repair the defective bone marrow-derived mesenchymal stromal cells in patients with systemic lupus erythematosus

被引：0

作者：

Xu, Jing-Jing ^{[1
]}

Sun, Yan-Bin ^{[2
]}

Zhang, Xiao-Li ^{[1
]}

Wang, Xiao-Fei ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Rheumatol, Shenyang 110004, Liaoning Provin, Peoples R China

[2] China Med Univ, Hosp 1, Dept Thorac Surg, Shenyang 110001, Liaoning Provin, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2015年 / 8卷 / 01期

关键词：

Systemic lupus erythematosus; bone marrow mesenchymal stem cells; EB1089; proliferation; Smad signaling pathway; STEM-CELLS; TERMINAL DIFFERENTIATION; CANCER CELLS; EB; 1089; APOPTOSIS; HORMONE; ACID;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Systemic lupus erythematosus (SLE) involves multiple factors, which result in the breakdown of self-tolerance and development of autoimmunity with organ damage. Bone marrow mesenchymal stem cells (BMMSCs) from the patients with SLE showed an impaired proliferative capacity compared with that from normal controls. In this study, we isolated BMMSCs from the patients with SLE and found that Vitamin D analog EB1089 could induce BMMSCs proliferation and mineralization deposition. Furthermore, we found that the expression of p-Smad 1/5/8 was promoted in BMMSCs with EB1089 treatment. In conclusion, our results support the notion that EB1089 promoted proliferation and osteogenic differentiation of BMMSCs by Smad 1/5/8 signaling pathway.

引用

页码：916 / 921

页数：6

共 20 条

[1] DIFFERENTIATION OF MOUSE MYELOID-LEUKEMIA CELLS INDUCED BY 1-ALPHA,25-DIHYDROXYVITAMIN-D3 [J].

ABE, E ;

MIYAURA, C ;

SAKAGAMI, H ;

TAKEDA, M ;

KONNO, K ;

YAMAZAKI, T ;

YOSHIKI, S ;

SUDA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (08) :4990-4994

[2] Mesenchymal stem cells are functionally abnormal in patients with immune thrombocytopenic purpura [J].

Antonio Perez-Simon, Jose ;

Tabera, Soraya ;

Eugenia Sarasquete, Maria ;

Diez-Campelo, Maria ;

Canchado, Javier ;

Ignacio Sanchez-Abarca, Luis ;

Blanco, Belen ;

Alberca, Ignacio ;

Herrero-Sanchez, Carmen ;

Canizo, Consuelo ;

San Miguel, Jesus F. .

CYTOTHERAPY, 2009, 11 (06) :698-705

[3] EB1089 inhibits the parathyroid hormone-related protein-enhanced bone metastasis and xenograft growth of human prostate cancer cells [J].

Bhatia, Vandanajay ;

Saini, Manjit K. ;

Shen, Xiaoli ;

Bi, Lian X. ;

Qiu, Suimin ;

Weigel, Nancy L. ;

Falzon, Miriam .

MOLECULAR CANCER THERAPEUTICS, 2009, 8 (07) :1787-1798

[4] Bone morphogenetic proteins [J].

Chen, D ;

Zhao, M ;

Mundy, GR .

GROWTH FACTORS, 2004, 22 (04) :233-241

[5] HETEROTOPIC TRANSPLANTS OF BONE MARROW - ANALYSIS OF PRECURSOR CELLS FOR OSTEOGENIC AND HEMATOPOIETIC TISSUES [J].

FRIEDENSTEIN, AJ ;

PETRAKOVA, KV ;

KUROLESOVA, AI ;

FROLOVA, GP .

TRANSPLANTATION, 1968, 6 (02) :230-+

[6] Terminal differentiation is not a major determinant for the success of stem cell therapy - cross-talk between muscle-derived stem cells and host cells [J].

Gharaibeh, Burhan ;

Lavasani, Mitra ;

Cummins, James H. ;

Huard, Johnny .

STEM CELL RESEARCH & THERAPY, 2011, 2

[7] Seocalcitol (EB 1089): A vitamin D analogue of anti-cancer potential. Background, design, synthesis, pre-clinical and clinical evaluation [J].

Hansen, CM ;

Hamberg, KJ ;

Binderup, E ;

Binderup, L .

CURRENT PHARMACEUTICAL DESIGN, 2000, 6 (07) :803-828

[8] EB 1089, a novel vitamin D analog with strong antiproliferative and differentiation-inducing effects on target cells [J].

Hansen, CM ;

Maenpaa, PH .

BIOCHEMICAL PHARMACOLOGY, 1997, 54 (11) :1173-1179

[9] REGULATION OF TERMINAL DIFFERENTIATION OF CULTURED MOUSE EPIDERMAL-CELLS BY 1-ALPHA,25-DIHYDROXYVITAMIN-D3 [J].

HOSOMI, J ;

HOSOI, J ;

ABE, E ;

SUDA, T ;

KUROKI, T .

ENDOCRINOLOGY, 1983, 113 (06) :1950-1957

[10]

Kamalaraj N, 2014, SCAND J RHEUMATOL, V7, P1

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