Molecular variation of hop mosaic virus isolates

被引:4
作者
Poke, Fiona S. [1 ,2 ]
Crowle, Damian R. [1 ]
Whittock, Simon P. [3 ,4 ]
Wilson, Calum R. [1 ]
机构
[1] Univ Tasmania, Tasmanian Inst Agr Res, Newtown, Tas 7008, Australia
[2] Univ Tasmania, Menzies Res Inst, Hobart, Tas 7000, Australia
[3] Univ Tasmania, Sch Plant Sci, Hobart, Tas 7001, Australia
[4] Hop Prod Australia, Bellerive, Tas 7018, Australia
关键词
HUMULUS-LUPULUS CULTIVARS; INFECTING HOP;
D O I
10.1007/s00705-010-0780-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hop mosaic virus (HpMV), a member of the genus Carlavirus, is importance to hop production worldwide. We identified variation in nucleic and amino acid sequences among 23 HpMV isolates from Australia, the USA, the Czech Republic, South Africa and Japan using a 1,455-bp fragment covering the 3' end of the virus genome including ORFs 4, 5 and 6. Three clusters of two or more isolates were identified in phylogenies of the total nucleotide sequence and the coat protein (ORF5) amino acid sequence. Two of these clusters combined in analyses of ORF4 and ORF6 amino acid sequences. Isolates from within and outside of Australia were found in each cluster, indicating that sequence variation was not associated with geographic source. Monitoring of HpMV variants in the field and evaluation of the impact of variants on vector association, rate of spread, and hop yield and quality can now be undertaken.
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页码:1721 / 1724
页数:4
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