Inhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis

被引：22
作者：
Chen, Shuang [1 ]
Yan, Jie [2 ]
Deng, Hai-xiao [3 ]
Long, Ling-ling [2 ]
Hu, Yong-jun [4 ]
Wang, Mi [1 ]
Shang, Lei [5 ]
Chen, Dan [1 ]
Huang, Ju-fang [1 ]
Xiong, Kun [1 ]
机构：
[1] Cent S Univ, Dept Anat & Neurobiol, Sch Basic Med Sci, Changsha 410013, Hunan, Peoples R China
[2] Cent S Univ, Dept Forens Sci, Sch Basic Med Sci, Changsha 410013, Hunan, Peoples R China
[3] Cent S Univ, Xiangya Sch Med, Year Med Program 5, Grade 2013, Changsha 410013, Hunan, Peoples R China
[4] Peoples Hosp Hunan Prov, Dept Cardiol, Changsha 410008, Hunan, Peoples R China
[5] Nanchang Univ, Affiliated Eye Hosp, Jiangxi Res Inst Ophthalmol & Visual Sci, Nanchang 330006, Peoples R China
来源：
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2016年 / 36卷 / 05期
基金：
中国国家自然科学基金;
关键词：
oxygen glucose deprivation; necroptosis; calpain; retinal ganglion cell-5; N-acetyl-leucyl-leucyl-norleucinal; truncated apoptosis inducing factor; CASPASE-INDEPENDENT NECROPTOSIS; ELEVATED HYDROSTATIC-PRESSURE; TNF-INDUCED NECROPTOSIS; RAT CORTICAL-NEURONS; CELL-DEATH; PROGRAMMED NECROSIS; HYDROGEN-PEROXIDE; INDUCED APOPTOSIS; INJURY; EXPRESSION;
D O I：
10.1007/s11596-016-1639-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco's-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
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页码：639 / 645
页数：7
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