Olfactory ensheathing cells conditioned medium prevented apoptosis induced by 6-OHDA in PC12 cells through modulation of intrinsic apoptotic pathways

被引：26

作者：

Feng, Lei ^{[1
]}

Meng, Haiwei ^{[1
]}

Wu, Fengxia ^{[1
]}

Cheng, Baohua ^{[1
]}

He, Xiuquan ^{[1
]}

Wang, Xiaohong ^{[1
]}

Li, Zhengping ^{[1
]}

Liu, Shuwei ^{[1
]}

机构：

[1] Shandong Univ, Sch Med, Dept Anat, Jinan 250012, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE | 2008年 / 26卷 / 3-4期

关键词：

olfactory ensheathing cells; PC12; cells; 6-OHDA; apoptosis; mitochondrial membrane potential; Bcl-2; Bax;

D O I：

10.1016/j.ijdevneu.2008.01.003

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Olfactory ensheathing cells (OECs) express a high level of growth factors which play a very important role as neuronal support. Recent evidence in literatures showed that transplantation of OECs may improve functional restoration in 6-OHDA-induced rat model of Parkinson's disease (PD). However, the biological function of various factors released from OECs in Parkinson' disease is still unclear. In this study, we examined the effects of newborn rat OECs conditioned medium (CM) on PC12 cells. Cells treated with 6-OHDA underwent cytotoxicity and apoptotic death determined by MTT assay and Hoechst 33342/PI staining. OECs CM was able to reduce the cellular damage in PC12 cells. Further investigation results showed that CM inhibited the disruption of mitochondrial transmembrane potential, up-regulation of Bcl-2 and downregulation of Bax. Taken together, this study indicates that CM has a neuroprotective effect on 6-OHDA induced apoptosis of PC12 cells, which is through up-regulation of the Bcl-2/Bax ratio and protection for mitochondrion. (C) 2008 ISDN. Published by Elsevier Ltd. All rights reserved.

引用

页码：323 / 329

页数：7

共 39 条

[1] Olfactory ensheathing cell transplantation restores functional deficits in rat model of Parkinson's disease: a cotransplantation approach with fetal ventral mesencephalic cells [J].

Agrawal, AK ;

Shukla, S ;

Chaturvedi, RK ;

Seth, K ;

Srivastava, N ;

Ahmad, A ;

Seth, PK .

NEUROBIOLOGY OF DISEASE, 2004, 16 (03) :516-526

[2] Identification of a human olfactory ensheathing cell that can effect transplant-mediated remyelination of demyelinated CNS axons [J].

Barnett, SC ;

Alexander, CL ;

Iwashita, Y ;

Gilson, JM ;

Crowther, J ;

Clark, L ;

Dunn, LT ;

Papanastassiou, V ;

Kennedy, PGE ;

Franklin, RJM .

BRAIN, 2000, 123 :1581-1588

[3] Olfactory ensheathing cells: Unique glial cell types? [J].

Barnett, SC .

JOURNAL OF NEUROTRAUMA, 2004, 21 (04) :375-382

[4] Extracellular toxicity of 6-hydroxydopamine on PC12 cells [J].

Blum, D ;

Torch, S ;

Nissou, MF ;

Benabid, AL ;

Verna, JM .

NEUROSCIENCE LETTERS, 2000, 283 (03) :193-196

[5] Olfactory ensheathing cells promote neurite sprouting of injured axons in vitro by direct cellular contact and secretion of soluble factors [J].

Chung, RS ;

Woodhouse, A ;

Fung, S ;

Dickson, TC ;

West, AK ;

Vickers, JC ;

Chuah, MI .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2004, 61 (10) :1238-1245

[6] The BCL2 family: Regulators of the cellular life-or-death switch [J].

Cory, S ;

Adams, JM .

NATURE REVIEWS CANCER, 2002, 2 (09) :647-656

[7] Olfactory ensheathing cells, olfactory nerve fibroblasts and biomatrices to promote long-distance axon regrowth and functional recovery in the dorsally hemisected adult rat spinal cord [J].

Deumens, R. ;

Koopmans, G. C. ;

Honig, W. M. M. ;

Hamers, F. P. T. ;

Maquet, V. ;

Jerome, R. ;

Steinbusch, H. W. M. ;

Joosten, E. A. J. .

EXPERIMENTAL NEUROLOGY, 2006, 200 (01) :89-103

[8]

Fiskum G, 2003, ANN NY ACAD SCI, V991, P111

[9]

Glinka Y, 1997, J NEURAL TRANSM-SUPP, P55

[10] Pathological synchronization in Parkinson's disease: networks, models and treatments [J].

Hammond, Constance ;

Bergman, Hagai ;

Brown, Peter .

TRENDS IN NEUROSCIENCES, 2007, 30 (07) :357-364

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