Emergence and genetic variability of Anaplasma species in small ruminants and ticks from Central Europe

被引：46

作者：

Derdakova, Marketa ^{[1
,2
]}

Stefancikova, Asteria ^{[1
]}

Spitalska, Eva ^{[3
]}

Taragel'ova, Veronika ^{[2
]}

Kost'alova, Tatiana ^{[4
]}

Hrkl'ova, Gabriela ^{[5
]}

Kybicova, Katerina ^{[6
]}

Schanilec, Pavel ^{[7
]}

Majlathova, Viktoria ^{[1
]}

Varady, Marian ^{[1
]}

Pet'ko, Branislav ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Zool, Bratislava 84506, Slovakia

[2] Slovak Acad Sci, Inst Parasitol, Kosice, Slovakia

[3] Slovak Acad Sci, Inst Virol, Bratislava 84506, Slovakia

[4] Charles Univ Prague, Prague, Czech Republic

[5] Catholic Univ, Ruzomberok, Slovakia

[6] Natl Inst Publ Hlth, Prague, Czech Republic

[7] Univ Vet & Pharmaceut Sci, Brno, Czech Republic

来源：

VETERINARY MICROBIOLOGY | 2011年 / 153卷 / 3-4期

关键词：

Anaplasma phagocytophilum; Anaplasma ovis; Ixodes ricinus; Dermacentor reticulatus; Dermacentor marginatus; Anaplasmosis; BORRELIA-BURGDORFERI; MOLECULAR-DETECTION; SEQUENCE-ANALYSIS; MSP4; GENE; PHAGOCYTOPHILUM; INFECTION; ANIMALS; OVIS; PREVALENCE; EHRLICHIA;

D O I：

10.1016/j.vetmic.2011.05.044

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Anaplasmoses are common tick-borne zoonotic bacterial diseases of livestock and free-living ungulates from the genus Anaplasma that are recently emerging in Central Europe. The main aim of this study was to analyze the prevalence and genetic variability of Anaplasma phagocytophilum and Anaplasma ovis in small ruminants and questing ticks from six different sites in Slovakia and the Czech Republic using the PCR of the msp4 gene followed by the sequence analysis. At two farms from southeastern Slovakia, 66.1% small ruminants were infected with A. ovis in contrast to one positive animal from both sites in northern Slovakia. It was represented by two different genotypes. A. phagocytophilum was present in all tested flocks with the infection prevalence ranging from 0.9% to 5.7%. None of the tested questing ticks carried A. ovis. A. phagocytophilum was detected in 1.1% and 7.8% of questing Ixodes ricinus ticks collected around the farms located in southeastern and northern Slovakia, respectively. A. phagocytophilum revealed higher intraspecific diversity than A. ovis. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：293 / 298

页数：6

共 29 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].