Simple way of pinpointing the three-dimensional position of biomarkers in fluorescence microscopy using a through-focus exposure method

被引：0

作者：

Kishima, Koichiro ^{[1
]}

机构：

[1] Sony Corp, Core Device Dev Grp, LE Business Dev Dept, Shinagawa Ku, Tokyo 1410001, Japan

来源：

APPLIED OPTICS | 2011年 / 50卷 / 25期

关键词：

DIFFRACTION-LIMIT; FIELD; RESOLUTION; DEPTH;

D O I：

10.1364/AO.50.004989

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The author proposes a method to identify the three-dimensional positions of fluorescent biomarkers by recording just two images. In the proposed method, the x and y positions of all fluorescent markers are recorded in the first exposure, and the z positions are obtained from a blurred image in the second exposure. The author has verified this method using a specimen with 1 mu m deep grooves and applied it to measuring chromatic aberration and the separation between two biological probes in fluorescence in situ hybridization cells. The method offers the advantage of greatly reduced data storage requirements. (C) 2011 Optical Society of America

引用

页码：4989 / 4997

页数：9

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