Isolation and Purification of Isochlorogenic Acid A from Lonicera japonica Thunb Using High-Speed Counter-Current Chromatography

被引：2

作者：

Yu, X. X. ^{[1
,2
]}

Wu, J. W. ^{[1
,2
]}

Liang, Z. K. ^{[1
,2
]}

Zhao, M. Q. ^{[1
,2
]}

Xu, X. J. ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou Higher Educ Mega Ctr, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangdong Technol Res Ctr Adv Chinese Med, Guangzhou 510006, Guangdong, Peoples R China

来源：

ACTA CHROMATOGRAPHICA | 2015年 / 27卷 / 03期

关键词：

Lonicera japonica Thunb; isochlorogenic acid A; HSCCC;

D O I：

10.1556/AChrom.27.2015.3.10

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-speed counter-current chromatography (HSCCC) method was established for the isolation and purification of isochlorogenic acid A from Lonicera japonica Thunb. The two-phase solvent system was composed of n-hexane: ethyl acetate: isopropanol: water (2:3:2:5, v/v/v/v). From 150 mg of the ethyl acetate fraction of L. japonica Thunb, 19.65 mg of isochlorogenic acid A was obtained in a one-step HSCCC separation, with a purity of 99.1%, as determined by high-performance liquid chromatography (HPLC). The structure was further identified by ultraviolet (UV), mass spectrometry (MS) and nuclear magnetic resonance (NMR).

引用

页码：541 / 549

页数：9

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