Coordinative control of G2/M phase of the cell cycle by non-coding RNAs in hepatocellular carcinoma

被引：15

作者：

Shi, Jun ^{[1
]}

Ye, Guangqiang ^{[2
]}

Zhao, Guoliang ^{[2
]}

Wang, Xuedong ^{[1
]}

Ye, Chunhui ^{[2
]}

Thammavong, Keooudone ^{[2
]}

Xu, Jing ^{[2
]}

Dong, Jiahong ^{[1
]}

机构：

[1] Tsinghua Univ, Sch Clin Med, Beijing Tsinghua Changguang Hosp, Dept Hepatobiliary & Pancreas Surg, Beijing, Peoples R China

[2] Guangxi Med Univ, Affiliated Hosp 1, Nanning, Peoples R China

来源：

PEERJ | 2018年 / 6卷

基金：

中国国家自然科学基金;

关键词：

Hepatocellular carcinoma cells; Microarray; miRNA; lncRNA; mRNA; CANCER STATISTICS; GENE; EXPRESSION; MITOSIS; MYT1;

D O I：

10.7717/peerj.5787

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objective: To investigate the interaction of non-coding RNAs (ncRNAs) in hepatocellular carcinoma. Methods: We compared the ncRNAs and mRNAs expression profiles of hepatocellular carcinoma and adjacent tissue by microarray and RT-PCR. The relationship between different ncRNAs and mRNA was analyzed using bioinformatics tools. A regulatory model of ncRNAs in hepatocellular carcinoma cells was developed. Results: A total of 1,704 differentially expressed lncRNAs, 57 miRNAs, and 2,093 mRNAs were identified by microarray analyses. There is a co-expression relationship between two ncRNAs (miRNA-125b-2-3p and lncRNA P26302). Bioinformatics analysis demonstrated cyclin-dependent kinases 1 and CyclinA2 as potential targets of miR-125b-2-3p and Polo-like kinase 1 as potential target of lncRNAP26302. All three gene are important components in the G2/M phase of cell cycle. Subsequently real-time polymerase chain reaction (PCR) studies confirmed these microarray results. Conclusion: MiR-125b-2-3p and lncRNAP26302 may affect the G2/M phase of the cell cycle through the regulation of their respective target genes. This study shows a role of ncRNAs in pathogenesis of hepatocellular carcinoma at molecular level, providing a basis for the future investigation aiming at early diagnosis and novel treatment of hepatocellular carcinoma.

引用

页数：11

共 29 条

[11] Mitotic degradation of cyclin A is mediated by multiple and novel destruction signals [J].

Kaspar, M ;

Dienemann, A ;

Schulze, C ;

Sprenger, F .

CURRENT BIOLOGY, 2001, 11 (09) :685-690

[12] Cyclin A-Cdk1 regulates the origin firing program in mammalian cells [J].

Katsuno, Yuko ;

Suzuki, Ayumi ;

Sugimura, Kazuto ;

Okumura, Katsuzumi ;

Zineldeen, Doaa H. ;

Shimada, Midori ;

Niida, Hiroyuki ;

Mizuno, Takeshi ;

Hanaoka, Fumio ;

Nakanishi, Makoto .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (09) :3184-3189

[13] Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells [J].

Lindon, C ;

Pines, J .

JOURNAL OF CELL BIOLOGY, 2004, 164 (02) :233-241

[14] Non-coding RNA [J].

Mattick, JS ;

Makunin, IV .

HUMAN MOLECULAR GENETICS, 2006, 15 :R17-R29

[15] Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate [J].

Nakajima, H ;

Toyoshima-Morimoto, F ;

Taniguchi, E ;

Nishida, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (28) :25277-25280

[16]

Perdiguero E, 2004, CELL CYCLE, V3, P733

[17] A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language? [J].

Salmena, Leonardo ;

Poliseno, Laura ;

Tay, Yvonne ;

Kats, Lev ;

Pandolfi, Pier Paolo .

CELL, 2011, 146 (03) :353-358

[18] Cdk1 is sufficient to drive the mammalian cell cycle [J].

Santamaria, David ;

Barriere, Cedric ;

Cerqueira, Antonio ;

Hunt, Sarah ;

Tardy, Claudine ;

Newton, Kathryn ;

Caceres, Javier F. ;

Dubus, Pierre ;

Malumbres, Marcos ;

Barbacid, Mariano .

NATURE, 2007, 448 (7155) :811-U8

[19] Long non-coding RNAs: A new frontier in the study of human diseases [J].

Shi, Xuefei ;

Sun, Ming ;

Liu, Hongbing ;

Yao, Yanwen ;

Song, Yong .

CANCER LETTERS, 2013, 339 (02) :159-166

[20] Polo-like kinase 1 reaches beyond mitosis-cytokinesis, DNA damage response, and development [J].

Takaki, Tohru ;

Trenz, Kristina ;

Costanzo, Vincenzo ;

Petronczkil, Mark .

CURRENT OPINION IN CELL BIOLOGY, 2008, 20 (06) :650-660

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