On-line Redox Derivatization Liquid Chromatography Using a Carbon Monolithic Column

被引：0

作者：

Miyashita, Ken ^{[1
,7
]}

Tanaka, Ryo ^{[1
]}

Hasegawa, George ^{[2
]}

Nakanishi, Kazuki ^{[3
]}

Morioka, Kazuhiro ^{[4
]}

Zeng, Hulie ^{[1
]}

Kato, Shungo ^{[1
]}

Uchiyama, Katsumi ^{[1
]}

Saitoh, Kazunori ^{[5
]}

Shibukawa, Masami ^{[6
]}

Nakajima, Hizuru ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Appl Chem Environm, Grad Sch Urban Environm Sci, 1-1 Minamiohsawa, Hachioji, Tokyo 1920397, Japan

[2] Kyushu Univ, Dept Appl Chem, Grad Sch Engn, Nishi Ku, 744 Motooka, Fukuoka, Fukuoka 8190395, Japan

[3] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto, Kyoto 6068502, Japan

[4] Tokyo Univ Pharm & Life Sci, Sch Pharm, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan

[5] Nihon Univ, Dept Appl Mol Chem, Coll Ind Technol, 1-2-1 Izumi Cho, Narashino, Chiba 2758575, Japan

[6] Saitama Univ, Grad Sch Sci & Technol, Dept Appl Chem, Sakura Ku, 255 Shimookubo, Saitama, Saitama 3388570, Japan

[7] GL Sci Inc, Shinjuku Ku, Shinjuku Sq Tower 30F,6-22-1 Nishi Shinjuku, Tokyo 1631130, Japan

来源：

BUNSEKI KAGAKU | 2018年 / 67卷 / 08期

关键词：

HPLC; on-line redox derivatization; porous graphitic carbon; carbon monolith; POROUS GRAPHITIC CARBON; BASIC SURFACE OXIDES; SEPARATION SELECTIVITY; ENHANCEMENT; COMPLEXES; POTENTIALS; PARAMETERS;

D O I：

10.2116/bunsekikagaku.67.469

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A macroporous resorcinol-formaldehyde (RF) xerogel was synthesized via sol gel reaction, accompanied by phase separation; a graphitized carbon monolith was then prepared by calcining the RF xerogel at a relatively low temperature (1200 degrees C) utilizing catalytic graphitization. The retention behaviors of metal-EDTA complexes on the carbon monolithic column were investigated. The carbon monolith exhibited catalytic activity for redox reactions, like porous graphitic carbon packing materials do; they oxidized Co(II)-EDTA to Co(III)-EDTA during elution. Their redox activity could be modified by treating with an eluent containing an oxidizing or reducing agent. A novel on-line redox derivatization HPLC system using a carbon monolithic column was developed for enhancing the separation selectivity. This on-line rcdox derivatization HPLC system consisted of two ODS columns as a separation column and one carbon monolithic column as redox derivatization unit placed between them. The Co(II)-EDTA migrated as its original oxidation state in the first ODS column, while it migrated as Co(III)-EDTA in the second ODS column, since Co(II)-EDTA was rapidly oxidized to Co(III)-EDTA in the carbon monolithic column. On the other hand, other metal-EDTA complexes migrated as their original oxidation states in these columns. Therefore, the Co-EDTA could be separated from the other metal-EDTA complexes. This on-line redox derivatization HPLC system was successfully used for determining the trace amount of cobalt in a reference copper alloy.

引用

页码：469 / 478

页数：10