Effect of Selected Phytochemicals on Cell Proliferation in A549 Lung Cancer Cells

被引：4

作者：

Yoon, Hyungeun ^{[1
]}

Lee, Chang Yong ^{[2
]}

机构：

[1] Sungshin Womens Univ, Dept Food & Nutr, Seoul 136742, South Korea

[2] Cornell Univ, Dept Food Sci & Technol, Geneva, NY 14456 USA

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2010年 / 19卷 / 04期

关键词：

lung cancer; A549; cell; quercetin; 3-beta-D-glucoside; resveratrol; curcumin; NF-KAPPA-B; GROWTH-INHIBITION; BREAST-CANCER; APOPTOSIS; ACTIVATION; EXPRESSION; RESVERATROL; RELEASE; CYCLE;

D O I：

10.1007/s10068-010-0149-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Effects of quercetin 3-beta-D-glucoside, resveratrol, and curcumin on A549 lung cancer cell proliferation and the mechanism of these phytochemicals in regulating apoptosis and cell cycle arrest were investigated. A549 cells were treated with quercetin 3-beta-D-glucoside, resveratrol, or curcumin at 37 degrees C for 96 hr and cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MIT) assay. Proteins related to apoptosis and cell cycle in A549 cells were quantified with Western blotting assay. Quercetin 3-beta-D-glucoside, resveratrol, and curcumin inhibited A549 cell proliferation in a dose-dependent manner (p < 0.05). Quercetin 3-beta-D-glucoside significantly decrease the expression level of CDK4 at concentrations of 5 mM and above (p < 0.05). Curcumin lowered expression level of Bcl-2, CDK4, and cyclin D1 at concentrations of 100, 50, and above, and 50 mM and above, respectively (p < 0.05). These results suggest that phytochemicals, which can be found in a normal diet, inhibit lung cancer cell proliferation and regulate the expression of the proteins involved in apoptosis and cell cycle.

引用

页码：1063 / 1068

页数：6

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