Embryogenic callus induction and Agrobacterium-mediated genetic transformation of 'Shine Muscat' grape

被引：10

作者：

Nakajima, Ikuko ^{[1
]}

Endo, Masaki ^{[2
]}

Haji, Takashi ^{[1
]}

Moriguchi, Takaya ^{[1
,3
]}

Yamamoto, Toshiya ^{[1
,4
]}

机构：

[1] Natl Agr & Food Res Org, Inst Fruit Tree & Tea Sci, Tsukuba, Ibaraki 3058605, Japan

[2] Natl Agr & Food Res Org, Inst Agrobiol Sci, Tsukuba, Ibaraki 3058605, Japan

[3] Shizuoka Profess Univ Agr, Shizuoka 4380803, Japan

[4] Natl Agr & Food Res Org, Dept Intellectual Property, Tsukuba, Ibaraki 3058517, Japan

来源：

PLANT BIOTECHNOLOGY | 2020年 / 37卷 / 02期

关键词：

Agrobacterium; embryogenic callus; genetic transformation; 'Shine Muscat' grape; VITIS-VINIFERA L; SOMATIC EMBRYOGENESIS; TARGETED MUTAGENESIS; THOMPSON SEEDLESS; TRANSGENIC PLANTS; ESTABLISHMENT; REGENERATION; RESISTANCE; CULTIVARS; CULTURES;

D O I：

10.5511/plantbiotechnology.20.0527a

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar 'Shine Muscat' (Vitis labruscana). Embryogenic calli were induced using flower bud filaments from a dormant cane. Agrobacterium strain LBA4404 harboring the binary plasmid pBin19-sgfp, which contains the sgfp and nptII genes, was used to infect embryogenic calli. Infected calli were selected on 1/2 MS medium containing 5% maltose and 2% agar supplemented with 15 mg l(-1) kanamycin. Efficiency of transformation of regenerated plants reached nearly 100% as determined by PCR and Southern blot analyses. The developed method will open a new avenue for genome editing of 'Shine Muscat' and contribute to the advancement of grape breeding.

引用

页码：185 / 194

页数：10

共 56 条

[1]

Bailey L. H., 1930, Hortus: a concise dictionary of gardening, general horticulture and cultivated plants in North America

[2]

Bouquet Alain, 2006, V344, P273

[3] An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.) [J].

Das, DK ;

Reddy, MK ;

Upadhyaya, KC ;

Sopory, SK .

PLANT CELL REPORTS, 2002, 20 (11) :999-1005

[4] Transgenic plants from shoot apical meristems of Vitis vinifera L. "Thompson Seedless" via Agrobacterium-mediated transformation [J].

Dutt, M. ;

Li, Z. T. ;

Dhekney, S. A. ;

Gray, D. J. .

PLANT CELL REPORTS, 2007, 26 (12) :2101-2110

[5]

Einset J., 1975, Advances in fruit breeding. Temperate fruits., P130

[6] Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation [J].

Fan, Di ;

Liu, Tingting ;

Li, Chaofeng ;

Jiao, Bo ;

Li, Shuang ;

Hou, Yishu ;

Luo, Keming .

SCIENTIFIC REPORTS, 2015, 5

[7] Green fluorescent protein as a screenable marker to increase the efficiency of generating transgenic woody fruit plants [J].

Ghorbel, R ;

Juárez, J ;

Navarro, L ;

Peña, L .

THEORETICAL AND APPLIED GENETICS, 1999, 99 (1-2) :350-358

[8]

HIRABAYASHI T, 1982, PLANT TISSUE CULTURE, P547

[9] NEW AGROBACTERIUM HELPER PLASMIDS FOR GENE-TRANSFER TO PLANTS [J].

HOOD, EE ;

GELVIN, SB ;

MELCHERS, LS ;

HOEKEMA, A .

TRANSGENIC RESEARCH, 1993, 2 (04) :208-218

[10]

Hoshino Y, 1998, PLANT BIOTECHNOL, V15, p29

← 1 2 3 4 5 6 →