Candida albicans DBF4 gene inducibly duplicated by the mini-Ura-blaster is involved in hypha-suppression

被引：9

作者：

Chien, Ting ^{[1
]}

Tseng, Tzu-Ling ^{[1
]}

Wang, Jiun-Yuan ^{[1
]}

Shen, Yi-Ting ^{[1
]}

Lin, Ting-Hui ^{[1
]}

Shieh, Jia-Ching ^{[1
,2
]}

机构：

[1] Chung Shan Med Univ, Dept Biomed Sci, Taichung, Taiwan

[2] Chung Shan Med Univ Hosp, Dept Med Res, Taichung, Taiwan

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2015年 / 779卷

关键词：

DBF4; Mini-Ura-blaster induced duplication; Hypha-suppression; CANDIDA-ALBICANS GENE; CELL-CYCLE; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; CDC7; DISRUPTION; MORPHOGENESIS; EXPRESSION; KINASE; GROWTH;

D O I：

10.1016/j.mrfmmm.2015.06.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The opportunistic human fungal pathogen Candida albicans is a natural diploid that does not have a complete sexual cycle. The ability to switch between diverse cellular forms is important to its virulence. Here, we describe the characterization of the C. albicans DBF4 gene, a Saccharomyces cerevisiae homolog that encodes a regulatory subunit of Cdc7 kinase that is known to initiate DNA replication. We made a C. albicans strain, with one DBF4 allele deleted by the mini-Ura-blaster and the other controlled by a repressible promoter. We also found a third CaDBF4 copy that was later verified to be inducibly duplicated by targeted recombination with the min-Ura-blaster. Surprisingly, the strain deleted with the third CaDBF4 copy exhibited hyphal growth under repressed conditions. We conclude that the CaDBF4 gene is prone to being duplicated by the mini-Ura-blaster and that it suppresses hyphal growth in C. albicans. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：78 / 85

页数：8

共 39 条

[1] Depletion of a polo-like kinase in Candida albicans activates cyclase-dependent hyphal-like growth [J].

Bachewich, C ;

Thomas, DY ;

Whiteway, M .

MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (05) :2163-2180

[2] Human zinc fingers as building blocks in the construction of artificial transcription factors [J].

Bae, KH ;

Do Kwon, Y ;

Shin, HC ;

Hwang, MS ;

Ryu, EH ;

Park, KS ;

Yang, HY ;

Lee, D ;

Lee, Y ;

Park, J ;

Kwon, HS ;

Kim, HW ;

Yeh, BI ;

Lee, HW ;

Sohn, SH ;

Yoon, J ;

Seol, W ;

Kim, JS .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :275-280

[3] UME6, a novel filament-specific regulator of Candida albicans hyphal extension and virulence [J].

Banerjee, Mohua ;

Thompson, Delma S. ;

Lazzell, Anna ;

Carlisle, Patricia L. ;

Pierce, Christopher ;

Monteagudo, Carlos ;

Lopez-Ribot, Jose L. ;

Kadosh, David .

MOLECULAR BIOLOGY OF THE CELL, 2008, 19 (04) :1354-1365

[4]

Becker D.M., 2001, CURR PROTOC MOL BIOL, V17

[5] The MET3 promoter:: a new tool for Candida albicans molecular genetics [J].

Care, RS ;

Trevethick, J ;

Binley, KM ;

Sudbery, PE .

MOLECULAR MICROBIOLOGY, 1999, 34 (04) :792-798

[6] Evaluation of the roles of four Candida albicans genes in virulence by using gene disruption strains that express URA3 from the native locus [J].

Cheng, SJ ;

Nguyen, MH ;

Zhang, ZD ;

Jia, HY ;

Handfield, M ;

Clancy, CJ .

INFECTION AND IMMUNITY, 2003, 71 (10) :6101-6103

[7] Dissection of the Candida albicans Cdc4 protein reveals the involvement of domains in morphogenesis and cell flocculation [J].

Chin, Chuen ;

Lai, Wei-Chung ;

Lee, Tai-Lin ;

Tseng, Tzu-Ling ;

Shieh, Jia-Ching .

JOURNAL OF BIOMEDICAL SCIENCE, 2013, 20

[8] Sensing the environment: Response of Candida albicans to the X factor [J].

Cottier, Fabien ;

Muhlschlegel, Fritz A. .

FEMS MICROBIOLOGY LETTERS, 2009, 295 (01) :1-9

[9] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI BY HIGH-VOLTAGE ELECTROPORATION [J].

DOWER, WJ ;

MILLER, JF ;

RAGSDALE, CW .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6127-6145

[10]

FONZI WA, 1993, GENETICS, V134, P717

← 1 2 3 4 →