Mid-Holocene changes in vegetation, palaeohydrology and climate were investigated from the sediments of Lake Vankavad in the northern taiga of the Usa Basin, NE European Russia, through the analysis of pollen, plant macrofossils, Cladocera and diatoms. Lake Vankavad was probably formed at ca. 5000 BP ( ca. 5600 cal. BP) and initially it was shallow with a littoral cladoceran fauna. Macrofossil and pollen results suggest that dense Betula-Picea forests grew in the vicinity and the shore was close to the sampling point. At ca. 4600 BP ( ca. 5400 cal. BP) the water level rose coincident with the decrease in the density and area of forests, probably caused by cooling climate and accelerated spread of mires. There was also a further rise in the water level at ca. 3500 BP ( ca. 3800 cal. BP). The initiation of the lake, followed by two periods of rising water-level, as well as the increase in mire formation, was a consequence of a rise in groundwater level. This probably reflects lower evapotranspiration in a cooling mid-Holocene climate and/or higher precipitation in the lowland area. Also the decreased forest density and area may have contributed to the lower evapotranspiration. It is also possible that permafrost aggradation or changes in peat ecosystems might have affected the hydrological conditions in the area.