Cyclooxygenase-2 up-regulation after FLAP transfection in human adenocarcinoma cell line HT29 cl.19A

Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 c1.19 A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 c1.19A represents an original mammalian model to study FLAP-dependent leukotriene synthesis. In FLAP cDNA transfected HT29 c1.19 A cells, FLAP expression led to an increase in cyclooxygenase pathway products (mainly PGE(2)) without an increase in 5-lipoxygenase metabolism. This increase in PGE2 synthesis was associated with a cyclooxygenase-2 upregulation in comparison to untransfected HT29 c1,19A cells. These results suggest a possible interaction between the two major pathways of arachidonic acid metabolism. (C) 1998 Federation of European Biochemical Societies.