Mapping the dynamics of shear stress-induced structural changes in endothelial cells

被引:71
|
作者
Mott, Rosalind E.
Helmke, Brian P.
机构
[1] Univ Virginia, Dept Biomed Engn, Charlottesville, VA 22908 USA
[2] Univ Virginia, Robert M Berne Cardiovasc Res Ctr, Charlottesville, VA USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2007年 / 293卷 / 05期
关键词
mechanotransduction; cytoskeleton; extracellular matrix; focal adhesion;
D O I
10.1152/ajpcell.00457.2006
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Hemodynamic shear stress regulates endothelial cell biochemical processes that govern cytoskeletal contractility, focal adhesion dynamics, and extracellular matrix (ECM) assembly. Since shear stress causes rapid strain focusing at discrete locations in the cytoskeleton, we hypothesized that shear stress coordinately alters structural dynamics in the cytoskeleton, focal adhesion sites, and ECM on a time scale of minutes. Using multiwavelength four-dimensional fluorescence microscopy, we measured the displacement of rhodamine-fibronectin and green fluorescent protein-labeled actin, vimentin, paxillin, and/or vinculin in aortic endothelial cells before and after onset of steady unidirectional shear stress. In the cytoskeleton, the onset of shear stress increased actin polymerization into lamellipodia, altered the angle of lateral displacement of actin stress fibers and vimentin filaments, and decreased centripetal remodeling of actin stress fibers in subconfluent and confluent cell layers. Shear stress induced the formation of new focal complexes and reduced the centripetal remodeling of focal adhesions in regions of new actin polymerization. The structural dynamics of focal adhesions and the fibronectin matrix varied with cell density. In subconfluent cell layers, shear stress onset decreased the displacement of focal adhesions and fibronectin fibrils. In confluent monolayers, the direction of fibronectin and focal adhesion displacement shifted significantly toward the downstream direction within 1 min after onset of shear stress. These spatially coordinated rapid changes in the structural dynamics of cytoskeleton, focal adhesions, and ECM are consistent with focusing of mechanical stress and/or strain near major sites of shear stress-mediated mechanotransduction.
引用
收藏
页码:C1616 / C1626
页数:11
相关论文
共 50 条
  • [31] Shear stress-induced endothelial gene expression is involved in the regulation of angiogenesis
    Zakrzewicz, A.
    Bongrazio, M.
    DaSilva-Azevedo, L.
    Chlench, S.
    Pohlkamp, Th
    Hoffmann, Ch
    Hohberg, M.
    Baum, O.
    Wunderlich, W.
    Alter, A.
    Knöchel, J.
    Pries, A. R.
    JOURNAL OF VASCULAR RESEARCH, 2006, 43 : 27 - 27
  • [32] ROS and NO Dynamics in Endothelial Cells Exposed to Exercise-Induced Wall Shear Stress
    Wang, Yan-Xia
    Liu, Hai-Bin
    Li, Peng-Song
    Yuan, Wen-Xue
    Liu, Bo
    Liu, Shu-Tian
    Qin, Kai-Rong
    CELLULAR AND MOLECULAR BIOENGINEERING, 2019, 12 (01) : 107 - 120
  • [33] ROS and NO Dynamics in Endothelial Cells Exposed to Exercise-Induced Wall Shear Stress
    Yan-Xia Wang
    Hai-Bin Liu
    Peng-Song Li
    Wen-Xue Yuan
    Bo Liu
    Shu-Tian Liu
    Kai-Rong Qin
    Cellular and Molecular Bioengineering, 2019, 12 : 107 - 120
  • [34] Microtubules and control of shear stress-induced endothelial cell motility and polarity
    McCue, SL
    Langille, BL
    MOLECULAR BIOLOGY OF THE CELL, 2004, 15 : 161A - 161A
  • [35] Role of cytoskeleton in shear stress-induced endothelial nitric oxide production
    Knudsen, HL
    Frangos, JA
    AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1997, 273 (01): : H347 - H355
  • [36] Stress-induced phase and structural changes in KDP crystals
    Mylvaganam, Kausala
    Zhang, Liangchi
    Zhang, Yong
    COMPUTATIONAL MATERIALS SCIENCE, 2015, 109 : 359 - 366
  • [37] β-arrestin is critical for early shear stress-induced Akt/eNOS activation in human vascular endothelial cells
    Carneiro, Ana Paula
    Fonseca-Alaniz, Miriam Helena
    Dallan, Luis Alberto Oliveira
    Miyakawa, Ayumi Aurea
    Krieger, Jose Eduardo
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 483 (01) : 75 - 81
  • [38] Shear stress-induced Ets-1 modulates protease inhibitor expression in microvascular endothelial cells
    Milkiewicz, Malgorzata
    Uchida, Cassandra
    Gee, Eric
    Fudalewski, Tomasz
    Haas, Tara L.
    JOURNAL OF CELLULAR PHYSIOLOGY, 2008, 217 (02) : 502 - 510
  • [39] Fluid shear stress-induced nitric oxide produation in human cavernosal endothelial cells: inhibition by hyperglycaemia
    Wessells, Hunter
    Teal, Thomas H.
    Engel, Karen
    Sullivan, Christopher J.
    Gallis, Byron
    Tran, Khoa B.
    Chitaley, Kanchan
    BJU INTERNATIONAL, 2006, 97 (05) : 1047 - 1052
  • [40] Spatiotemporal analysis of shear stress-induced calpain activations in cultured human umbilical vein endothelial cells
    Miyazaki, T
    Honda, K
    Ohata, H
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2006, 100 : 80P - 80P