Biodegradable fumarate-based polyHIPEs as tissue engineering scaffolds

被引:129
作者
Christenson, Elizabeth M. [1 ]
Soofi, Wafa [1 ]
Holm, Jennifer L. [1 ]
Cameron, Neil R. [2 ]
Mikos, Antonios G. [1 ]
机构
[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA
[2] Univ Durham, Dept Chem, Durham DH1 3LE, England
关键词
D O I
10.1021/bm7007235
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
PolyHIPEs show great promise as tissue engineering scaffolds due to the tremendous control of pore size and interconnectivity afforded by this technique. Highly porous, fully biodegradable scaffolds were prepared by polymerization of the continuous phase of high internal phase emulsions (HIPEs) containing the macromer poly(propylene fumarate) (PPF) and the cross-linker propylene fumarate diacrylate (PFDA). Toluene was used as a diluent to reduce the viscosity of the organic phase to enable HIPE formation. A range of polyHIPE scaffolds of different pore sizes and morphologies were generated by varying the diluent concentration (40-60 wt %), cross-linker concentration (25-75 wt %), and macromer molecular weight (M-n = 800-1000 g/mol). Although some formulations resulted in macroporous monoliths (pore diameter > 500 mu m), the majority of the polyHIPEs studied were rigid, microporous monoliths with average pore diameters in the range 10-300 mu m. Gravimetric analysis confirmed the porosity of the microporous monoliths as 80-89% with most scaffolds above 84%. These studies demonstrate that emulsion templating can be used to generate rigid, biodegradable scaffolds with highly interconnected pores suitable for tissue engineering scaffolds.
引用
收藏
页码:3806 / 3814
页数:9
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