DNA damage in rats after treatment with methylphenidate

被引：61
作者：
Andreazza, Ana Cristina
Frey, Benicio N.
Valvassori, Samira S.
Zanotto, Caroline
Gomes, Karin M.
Comim, Clarissa M.
Stertz, Laura
Ribeiro, Leticia C.
Quevedo, Joao
Kapczinski, Flavio
Berk, Michael
Goncalves, Carlos Alberto
机构：
[1] Hosp Clin Porto Alegre, Ctr Pesquisas, Bipolar Disorders Program, BR-90035003 Porto Alegre, RS, Brazil
[2] Hosp Clin Porto Alegre, Ctr Pesquisas, Lab Mol Psychiat, BR-90035003 Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Biochem, BR-90035003 Porto Alegre, RS, Brazil
[4] Univ Extremo Catarinense, Programa Posgrad & Ciencias Saude, BR-88806000 Criciuma, SC, Brazil
[5] Barwobn Hlth & Geelong Clin, Dept Psychiat, Geelong, Vic, Australia
[6] Mental Hlth Res Inst, Melbourne, Vic, Australia
[7] Orygen Res Ctr, Melbourne, Vic, Australia
来源：
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | 2007年 / 31卷 / 06期
关键词：
attention-deficit hyperactivity disorder; comet assay; DNA damage; methylphenidate; micronucleus test; oxidative stress;
D O I：
10.1016/j.pnpbp.2007.05.012
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
Background: Methylphenidate (MPH) is a widely prescribed psychostimulant for the treatment of attention-deficit hyperactivity disorder (ADHD). Recently, some studies have addressed the genotoxic potential of the MPH, but the results have been contradictory. Hence, the present study aimed to investigate the index of cerebral and peripheral DNA damage in young and adult rats after acute and chronic MPH exposure. Methods: We used (1) single cell gel electrophoresis (Comet assay) to measure early DNA damage in hippocampus, striatum and total blood, and (2) micronucleus test in total blood samples. Results: Our results showed that MPH increased the peripheral index of early DNA damage in young and adult rats, which was more pronounced with chronic treatment and in the striatum compared to the hippocampus. Neither acute nor chronic MPH treatment increased micronucleus frequency in young or in adult rats. Peripheral DNA damage was positively correlated with striatal DNA damage. Conclusion: These results suggest that MPH may induce central and peripheral early DNA damage, but this early damage may be repaired. (C) 2007 Elsevier Inc. All rights reserved.
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收藏
页码：1282 / 1288
页数：7
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