Rapid screening and characterization of glucosinolates in 25 Brassicaceae tissues by UHPLC-Q-exactive orbitrap-MS

被引：34
作者：
Dong, Meiyue [1 ]
Tian, Zhenhua [2 ]
Ma, Yanni [1 ]
Yang, Zhongyi [1 ]
Ma, Zhen [1 ]
Wang, Xiaoming [2 ,3 ,4 ]
Li, Yunlun [3 ,4 ,5 ]
Jiang, Haiqiang [2 ,3 ,4 ]
机构：
[1] Shandong Univ Tradit Chinese Med, Sch Pharm, Jinan 250355, Peoples R China
[2] Shandong Univ Tradit Chinese Med, Expt Ctr, Jinan 250355, Peoples R China
[3] Shandong Univ Tradit Chinese Med, Key Lab Tradit Chinese Med Class Theory, Minist Educ, Jinan 250355, Peoples R China
[4] Shandong Univ Tradit Chinese Med, Shandong Prov Key Lab Tradit Chinese Med Basic Re, Jinan 250355, Peoples R China
[5] Shandong Univ Tradit Chinese Med, TCM Clin Res Base Hypertens, Affiliated Hosp, Jinan 250011, Peoples R China
来源：
FOOD CHEMISTRY | 2021年 / 365卷
关键词：
Brassicaceae; Glucosinolates; UHPLC-Q-exactive orbitrap-MS; Mass defect filtering; Diagnostic ion filtering; RESOLUTION MASS-SPECTROMETRY; LINEAR ION-TRAP; ELECTROSPRAY-IONIZATION; ISOTHIOCYANATES; QUANTIFICATION; IDENTIFICATION; SEPARATION; VEGETABLES; SPROUTS; ROOTS;
D O I：
10.1016/j.foodchem.2021.130493
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Glucosinolates (GSLs) are secondary plant metabolites that occur mainly in the Brassicaceae plants, which are desirable compounds in human foods due to their diverse biological activities. In this study, we developed an integrated data filtering and identification strategy to characterize the GSLs. An in-depth GSLs profiling was performed on 25 commonly Brassicaceae tissues in Jinan, China. By comparison with the reference standards and previous researches, we tentatively identified 47 GSLs including 8 unknown ones. The GSLs profiles of 25 Brassicaceae tissues were established, and 11 markers of GSLs could be used to distinguish the Brassica and Raphanus. This approach enables accurately characterization the GSLs of Brassicaceae tissues, and demonstrates the potential of GSLs profiles for Brassicaceae species discrimination.
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页数：11
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