Rapid screening and characterization of glucosinolates in 25 Brassicaceae tissues by UHPLC-Q-exactive orbitrap-MS

被引：35

作者：

Dong, Meiyue ^{[1
]}

Tian, Zhenhua ^{[2
]}

Ma, Yanni ^{[1
]}

Yang, Zhongyi ^{[1
]}

Ma, Zhen ^{[1
]}

Wang, Xiaoming ^{[2
,3
,4
]}

Li, Yunlun ^{[3
,4
,5
]}

Jiang, Haiqiang ^{[2
,3
,4
]}

机构：

[1] Shandong Univ Tradit Chinese Med, Sch Pharm, Jinan 250355, Peoples R China

[2] Shandong Univ Tradit Chinese Med, Expt Ctr, Jinan 250355, Peoples R China

[3] Shandong Univ Tradit Chinese Med, Key Lab Tradit Chinese Med Class Theory, Minist Educ, Jinan 250355, Peoples R China

[4] Shandong Univ Tradit Chinese Med, Shandong Prov Key Lab Tradit Chinese Med Basic Re, Jinan 250355, Peoples R China

[5] Shandong Univ Tradit Chinese Med, TCM Clin Res Base Hypertens, Affiliated Hosp, Jinan 250011, Peoples R China

来源：

FOOD CHEMISTRY | 2021年 / 365卷

关键词：

Brassicaceae; Glucosinolates; UHPLC-Q-exactive orbitrap-MS; Mass defect filtering; Diagnostic ion filtering; RESOLUTION MASS-SPECTROMETRY; LINEAR ION-TRAP; ELECTROSPRAY-IONIZATION; ISOTHIOCYANATES; QUANTIFICATION; IDENTIFICATION; SEPARATION; VEGETABLES; SPROUTS; ROOTS;

D O I：

10.1016/j.foodchem.2021.130493

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Glucosinolates (GSLs) are secondary plant metabolites that occur mainly in the Brassicaceae plants, which are desirable compounds in human foods due to their diverse biological activities. In this study, we developed an integrated data filtering and identification strategy to characterize the GSLs. An in-depth GSLs profiling was performed on 25 commonly Brassicaceae tissues in Jinan, China. By comparison with the reference standards and previous researches, we tentatively identified 47 GSLs including 8 unknown ones. The GSLs profiles of 25 Brassicaceae tissues were established, and 11 markers of GSLs could be used to distinguish the Brassica and Raphanus. This approach enables accurately characterization the GSLs of Brassicaceae tissues, and demonstrates the potential of GSLs profiles for Brassicaceae species discrimination.

引用

页数：11

共 35 条

[1] Specific Glucosinolate Analysis Reveals Variable Levels of Epimeric Glucobarbarins, Dietary Precursors of 5-Phenyloxazolidine-2-thiones, in Watercress Types with Contrasting Chromosome Numbers [J].

Agerbirk, Niels ;

Olsen, Carl Erik ;

Cipollini, Don ;

Orgaard, Marian ;

Linde-Laursen, Ib ;

Chew, Frances S. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (39) :9586-9596

[2] Isoferuloyl derivatives of five seed glucosinolates in the crucifer genus Barbarea [J].

Agerbirk, Niels ;

Olsen, Carl Erik .

PHYTOCHEMISTRY, 2011, 72 (07) :610-623

[3] Investigation of Glucosinolate Profile and Qualitative Aspects in Sprouts and Roots of Horseradish (Armoracia rusticana) Using LC-ESI Hybrid Linear Ion Trap with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Infrared Multiphoton Dissociation [J].

Agneta, Rosa ;

Rivelli, Anna Rita ;

Ventrella, Emanuela ;

Lelario, Filomena ;

Sarli, Giulio ;

Bufo, Sabino Aurelio .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (30) :7474-7482

[4] Simultaneous Analysis of Glucosinolates and Isothiocyanates by Reversed-Phase Ultra-High-Performance Liquid Chromatography-Electron Spray Ionization-Tandem Mass Spectrometry [J].

Andini, Silvia ;

Araya-Cloutier, Carla ;

Sanders, Mark ;

Vincken, Jean-Paul .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 68 (10) :3121-3131

[5] Modulation of Glucosinolate Composition in Brassicaceae Seeds by Germination and Fungal Elicitation [J].

Andini, Silvia ;

Dekker, Pieter ;

Gruppen, Harry ;

Araya-Cloutier, Carla ;

Vincken, Jean-Paul .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (46) :12770-12779

[6] Extraction, profiling and bioactivity analysis of volatile glucosinolates present in oil extract of Brassica juncea var. raya [J].

Bassan, Priyanka ;

Bhushan, Sakshi ;

Kaur, Tajinder ;

Arora, Rohit ;

Arora, Saroj ;

Vig, Adarsh Pal .

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2018, 24 (03) :399-409

[7] Screening crucifer seeds as sources of specific intact glucosinolates using ion-pair high-performance liquid chromatography negative ion electrospray mass spectrometry [J].

Bennett, RN ;

Mellon, FA ;

Kroon, PA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (03) :428-438

[8] Comparison of Glucosinolate Profiles in Different Tissues of Nine Brassica Crops [J].

Bhandari, Shiva Ram ;

Jo, Jung Su ;

Lee, Jun Gu .

MOLECULES, 2015, 20 (09) :15827-15841

[9] Acylated glucosinolates with diverse acyl groups investigated by high resolution mass spectrometry and infrared multiphoton dissociation [J].

Bianco, Giuliana ;

Agerbirk, Niels ;

Losito, Ilario ;

Cataldi, Tommaso R. I. .

PHYTOCHEMISTRY, 2014, 100 :92-102

[10] Chromatographic column evaluation for the untargeted profiling of glucosinolates in cauliflower by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry [J].

Capriotti, Anna Laura ;

Cavaliere, Chiara ;

La Barbera, Giorgia ;

Montone, Carmela Maria ;

Piovesana, Susy ;

Chiozzi, Riccardo Zenezini ;

Lagana, Aldo .

TALANTA, 2018, 179 :792-802

← 1 2 3 4 →