Resonance Light Scattering study on the interaction of victory blue-B with deoxyribonucleic acid and application to DNA assay

被引：0

作者：

Su, JS ^{[1
]}

Chen, XM

Long, YF

Li, W

Luo, HT

机构：

[1] Xiangtan Univ, Inst Chem, Xiangtan 411105, Peoples R China

[2] Hunan Univ Sci & Technol, Dept Chem, Xiangtan 411201, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2004年 / 24卷 / 01期

关键词：

Victory blue-B; DNA; resonance scattering spectroscopy;

D O I：

暂无

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The enhancement of the Resonance Light Scattering (RLS) due to Victory blue-B on the molecular surface of DNA in alkalescence solution was studied. A method of resonance light scattering was developed to determine DNA. Deoxyribonucleic acid(DNA) was studied in the range of pH 9.0-10.5. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of DNA at nanogram level based on RLS measurement. The linear range of the DNA is 0-3.0 mg.L-1 with a correlation coefficient of 0.997 8. The detection limit is 21.5 mug(.)L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.

引用

页码：37 / 40

页数：4