Quantification of Squalene in Olive Oil Using 13C Nuclear Magnetic Resonance Spectroscopy

被引：17

作者：

Nam, Anne-Marie ^{[1
]}

Bighelli, Ange ^{[1
]}

Tomi, Felix ^{[1
]}

Casanova, Joseph ^{[1
]}

Paoli, Mathieu ^{[1
]}

机构：

[1] Univ Corse, CNRS, Equipe Chim & Biomasse, UMR 6134,SPE, Route Sanguinaires, F-20000 Ajaccio, France

来源：

MAGNETOCHEMISTRY | 2017年 / 3卷 / 04期

关键词：

squalene; olive oil; C-13 NMR analysis; quantification; Corsica; QUANTITATIVE-DETERMINATION; NMR-SPECTROSCOPY; LIQUID-CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; IDENTIFICATION; EXTRACTS; TOCOPHEROLS; TRITERPENES; FRACTION; STEROLS;

D O I：

10.3390/magnetochemistry3040034

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

In the course of our ongoing work on the chemical characterization of Corsican olive oil, we have developed and validated a method for direct quantification of squalene using C-13 Nuclear Magnetic Resonance (NMR) spectroscopy without saponification, extraction, or fractionation of the investigated samples. Good accuracy, linearity, and precision of the measurements have been observed. The experimental procedure was applied to the quantification of squalene in 24 olive oil samples from Corsica. Squalene accounted for 0.35-0.83% of the whole composition.

引用

页数：10

共 42 条

[1] High Resolution NMR Spectroscopy as a Structural and Analytical Tool for Unsaturated Lipids in Solution [J].

Alexandri, Eleni ;

Ahmed, Raheel ;

Siddiqui, Hina ;

Choudhary, Muhammad I. ;

Tsiafoulis, Constantinos G. ;

Gerothanassis, Ioannis P. .

MOLECULES, 2017, 22 (10)

[2] Multivariate analysis of NMR fingerprint of the unsaponifiable fraction of virgin olive oils for authentication purposes [J].

Alonso-Salces, R. M. ;

Heberger, K. ;

Holland, M. V. ;

Moreno-Rojas, J. M. ;

Mariani, C. ;

Bellan, G. ;

Reniero, F. ;

Guillou, C. .

FOOD CHEMISTRY, 2010, 118 (04) :956-965

[3]

Auffray B., 2007, International Journal of Cosmetic Science, V29, P23, DOI 10.1111/j.1467-2494.2007.00360.x

[4] SELECTION OF OPTIMUM PARAMETERS FOR PULSE FOURIER-TRANSFORM NUCLEAR MAGNETIC-RESONANCE [J].

BECKER, ED ;

FERRETTI, JA ;

GAMBHIR, PN .

ANALYTICAL CHEMISTRY, 1979, 51 (09) :1413-1420

[5] Identification and quantitative determination of eudesmane-type acids from the essential oil of Dittrichia viscosa sp viscosa using 13C-NMR spectroscopy [J].

Blanc, MC ;

Bradesi, P ;

Casanova, J .

PHYTOCHEMICAL ANALYSIS, 2005, 16 (03) :150-154

[6] SQUALENE RECOVERY FROM OLIVE OIL DEODORIZER DISTILLATES [J].

BONDIOLI, P ;

MARIANI, C ;

LANZANI, A ;

FEDELI, E ;

MULLER, A .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1993, 70 (08) :763-766

[7] Direct qualitative and quantitative analysis of triterpenes using 13C NMR spectroscopy exemplified by dichloromethanic extracts of cork [J].

Castola, V ;

Bighelli, A ;

Casanova, J .

APPLIED SPECTROSCOPY, 1999, 53 (03) :344-350

[8] Quality assessment and authentication of virgin olive oil by NMR spectroscopy: A critical review [J].

Dais, Photis ;

Hatzakis, Emmanuel .

ANALYTICA CHIMICA ACTA, 2013, 765 :1-27

[9] Using lipid profiles and genotypes for the characterization of Corsican olive oils [J].

de Caraffa, Virginie Bronzini ;

Gambotti, Claude ;

Giannettini, Jean ;

Maury, Jacques ;

Berti, Liliane ;

Gandemer, Gilles .

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2008, 110 (01) :40-47

[10]

De Leonardis A, 1998, ITAL J FOOD SCI, V10, P75

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