Identification of organophosphate modifications by high-resolution mass spectrometry

被引：8

作者：

Lee, Jun Hyung ^{[1
]}

Jang, Wooyoung Eric ^{[2
]}

Park, Ji Hwan ^{[1
]}

Mohammad, Hazara Begum ^{[1
]}

Lee, Jin-Young ^{[3
]}

Jeong, Woo-Hyeon ^{[3
]}

Kim, Min-Sik ^{[1
,2
]}

机构：

[1] DGIST, New Biol, Daegu 42988, South Korea

[2] Kyung Hee Univ, Dept Chem, Gyeonggi Do, South Korea

[3] Agcy Def Dev, Chembio Technol Ctr, Daejeon, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2022年 / 43卷 / 03期

关键词：

human plasma; mass spectrometry; nuerotoxicity; organophosphate; protein modification; HUMAN SERUM-ALBUMIN; NERVE AGENTS; EXPOSURE; ADDUCTS; PESTICIDES; BIOMARKER;

D O I：

10.1002/bkcs.12478

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Organophosphate (OP) compounds exhibit neurotoxicity by binding to serine residues of acetylcholinesterase (AChE) in the cholinergic nervous system and subsequently lead to the accumulation of acetylcholine in neuromuscular junctions of synapses. AChE capable of hydrolyzing choline esters is known to be inhibited in patients with nerve agents poisoning. Since OP is known to be transported by covalent bonding to human serum albumin (HSA), OP-HSA adducts are considered potential diagnostic markers for OP exposures. In this study, HSA modification sites by OP or OP-like compounds such as V-type (VX) and Novichok-type (A234), insecticide (DFP), and serine protease inhibitor (PMSF) were studied using liquid chromatography-high-resolution tandem mass spectrometry. As a result, we have discovered a novel OP-HSA modification site, Y341.

引用

页码：444 / 449

页数：6

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