Rapid hyaluronan uptake is associated with enhanced motility: implications for an intracellular mode of action

被引：94
作者：
Collis, L
Hall, C
Lange, L
Ziebell, M
Prestwich, R
Turley, EA
机构：
[1] Hosp Sick Children, Div Cardiovasc Res, Toronto, ON M5G 1X8, Canada
[2] Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada
[3] Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA
来源：
FEBS LETTERS | 1998年 / 440卷 / 03期
基金：
英国医学研究理事会;
关键词：
hyaluronan; RHAMM; CD44; internalization; lamella; nucleus;
D O I：
10.1016/S0014-5793(98)01505-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Texas red-labeled hyaluronan (TR-HA) is rapidly taken up in a CD44 independent manner into ras-transformed 10T1/2 fibroblasts, where it accumulates in both cell ruffles/ lamellae, the perinuclear area, and the nucleus. HA does not accumulate in the cell ruffles/lamellae of parental 10T1/2 cells. Addition of HA to ras-transformed cells promotes their random motility but has no effect on 10T1/2 cell motility. 10T1/2 cells can be modified to take up HA into cell ruffles by exposure to phorbol ester or direct microinjection of HA into cells. Both treatments significantly stimulate 10T1/2 cell motility, (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：444 / 449
页数：6
相关论文
共 48 条
[21]  
Graves LM, 1997, ADV SEC MESS PHOSPH, V31, P49
[22]   HYALURONAN AND THE HYALURONAN RECEPTOR RHAMM PROMOTE FOCAL ADHESION TURNOVER AND TRANSIENT TYROSINE KINASE-ACTIVITY [J].
HALL, CL ;
WANG, C ;
LANGE, LA ;
TURLEY, EA .
JOURNAL OF CELL BIOLOGY, 1994, 126 (02) :575-588
[23]   OVEREXPRESSION OF THE HYALURONAN RECEPTOR RHAMM IS TRANSFORMING AND IS ALSO REQUIRED FOR H-RAS TRANSFORMATION [J].
HALL, CL ;
YANG, BH ;
YANG, XW ;
ZHANG, SW ;
TURLEY, M ;
SAMUEL, S ;
LANGE, LA ;
WANG, C ;
CURPEN, GD ;
SAVANI, RC ;
GREENBERG, AH ;
TURLEY, EA .
CELL, 1995, 82 (01) :19-28
[24]  
HISCOCK DRR, 1994, J BIOL CHEM, V269, P4539
[25]  
HUA Q, 1993, J CELL SCI, V106, P365
[26]   Regulation of cell motility by mitogen-activated protein kinase [J].
Klemke, RL ;
Cai, S ;
Giannini, AL ;
Gallagher, PJ ;
deLanerolle, P ;
Cheresh, DA .
JOURNAL OF CELL BIOLOGY, 1997, 137 (02) :481-492
[27]   INTRACELLULAR SIGNAL-TRANSDUCTION FOR SERUM ACTIVATION OF THE HYALURONAN SYNTHASE IN EUKARYOTIC CELL-LINES [J].
KLEWES, L ;
PREHM, P .
JOURNAL OF CELLULAR PHYSIOLOGY, 1994, 160 (03) :539-544
[28]   HYALURONAN-BINDING PROTEINS IN DEVELOPMENT, TISSUE HOMEOSTASIS, AND DISEASE [J].
KNUDSON, CB ;
KNUDSON, W .
FASEB JOURNAL, 1993, 7 (13) :1233-1241
[29]   Interaction between Cdc37 and Cdk4 in human cells [J].
Lamphere, L ;
Fiore, F ;
Xu, X ;
Brizuela, L ;
Keezer, S ;
Sardet, C ;
Draetta, GF ;
Gyuris, J .
ONCOGENE, 1997, 14 (16) :1999-2004
[30]  
Laurent Torvard C., 1996, Immunology and Cell Biology, V74, pA1, DOI 10.1038/icb.1996.32
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