Rapid hyaluronan uptake is associated with enhanced motility: implications for an intracellular mode of action

被引：94

作者：

Collis, L

Hall, C

Lange, L

Ziebell, M

Prestwich, R

Turley, EA

机构：

[1] Hosp Sick Children, Div Cardiovasc Res, Toronto, ON M5G 1X8, Canada

[2] Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada

[3] Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA

来源：

FEBS LETTERS | 1998年 / 440卷 / 03期

基金：

英国医学研究理事会;

关键词：

hyaluronan; RHAMM; CD44; internalization; lamella; nucleus;

D O I：

10.1016/S0014-5793(98)01505-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Texas red-labeled hyaluronan (TR-HA) is rapidly taken up in a CD44 independent manner into ras-transformed 10T1/2 fibroblasts, where it accumulates in both cell ruffles/ lamellae, the perinuclear area, and the nucleus. HA does not accumulate in the cell ruffles/lamellae of parental 10T1/2 cells. Addition of HA to ras-transformed cells promotes their random motility but has no effect on 10T1/2 cell motility. 10T1/2 cells can be modified to take up HA into cell ruffles by exposure to phorbol ester or direct microinjection of HA into cells. Both treatments significantly stimulate 10T1/2 cell motility, (C) 1998 Federation of European Biochemical Societies.

引用

收藏

页码：444 / 449

页数：6

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