Porcine Circovirus (PCV) Genotype 2d-Based Virus-like Particles (VLPs) Induced Broad Cross-Neutralizing Antibodies against Diverse Genotypes and Provided Protection in Dual-Challenge Infection of a PCV2d Virus and a Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)

被引:24
作者
Kang, Seok-Jin [1 ]
Bae, Sung-Min [2 ]
Lee, Hye-Jeong [1 ]
Jeong, Young-Ju [2 ]
Lee, Min-A [1 ]
You, Su-Hwa [1 ]
Lee, Hyang-Sim [1 ]
Hyun, Bang-Hun [1 ]
Lee, Nakhyung [2 ]
Cha, Sang-Ho [1 ,3 ]
机构
[1] Anim & Plant Quarantine Agcy, Div Viral Dis, Gimcheon Si 39660, South Korea
[2] KBNP, Anyang Si 14059, South Korea
[3] Anim & Plant Quarantine Agcy, Div Foot & Mouth Dis Res, Gimcheon Si 39660, South Korea
关键词
porcine circovirus type 2; recombinant VLP; vaccine; cross-neutralization; VACCINE; PIGS; 2B; CLUSTER; US;
D O I
10.3390/pathogens10091145
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
As PCV2d infection has been continuously reported in swine farms in which pigs were vaccinated with PCV2a- or 2d-based vaccines, we attempted to develop a novel vaccine using a PCV2d-based capsid to enhance its protective efficacy. In this study, recombinant virus-like particles (VLPs) of rPCV2a, rPCV2b and rPCV2d were synthesized from the capsid proteins of PCV2a, PCV2b and PCV2d field isolates, respectively. A cross-neutralization assay between the VLPs induced antisera and the field isolates demonstrated the broad cross-neutralizing activities of the rPCV2d-induced antisera. Then, the protective efficacy of rPCV2d as a vaccine candidate was investigated in commercial pigs by rPCV2d vaccination and a single- or dual-challenge infection using a PCV2d strain and a type 1 PRRSV strain. High levels of anti-PCV2d IgG and neutralizing antibodies were induced 3 weeks after vaccination. After the challenge infection, the average ADWG values of the vaccinated group were higher than those of the unvaccinated group. None or a significantly low amount of (p < 0.05) reduced PCV2 genomic DNA was found in the blood, saliva and tissues of the vaccinated pigs, when compared to the unvaccinated group. Moreover, macroscopic and microscopic lesions in the tissues were significantly (p < 0.05) reduced in the vaccinated groups. This study therefore suggests that rPCV2d may be highly useful for the control of diverse field genotypes.
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页数:13
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共 33 条
[1]   Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication [J].
Allan, GM ;
McNeilly, F ;
Ellis, J ;
Krakowka, S ;
Meehan, B ;
McNair, I ;
Walker, I ;
Kennedy, S .
ARCHIVES OF VIROLOGY, 2000, 145 (11) :2421-2429
[2]   An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome [J].
Blanchard, P ;
Mahé, D ;
Cariolet, R ;
Truong, C ;
Le Dimna, M ;
Arnauld, C ;
Rose, N ;
Eveno, E ;
Albina, E ;
Madec, F ;
Jestin, A .
VETERINARY MICROBIOLOGY, 2003, 94 (03) :183-194
[3]   Recombinant protein vaccines produced in insect cells [J].
Cox, Manon M. J. .
VACCINE, 2012, 30 (10) :1759-1766
[4]   Virus-like particles: The new frontier of vaccines for animal viral infections [J].
Crisci, Elisa ;
Barcena, Juan ;
Montoya, Maria .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2012, 148 (3-4) :211-225
[5]   Detection of a new cluster of porcine circovirus type 2b strains in domestic pigs in Germany [J].
Eddicks, M. ;
Fux, R. ;
Szikora, F. ;
Eddicks, L. ;
Majzoub-Altweck, M. ;
Hermanns, W. ;
Sutter, G. ;
Palzer, A. ;
Banholzer, E. ;
Ritzmann, M. .
VETERINARY MICROBIOLOGY, 2015, 176 (3-4) :337-343
[6]   Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs [J].
Fort, Maria ;
Olvera, Alex ;
Sibila, Marina ;
Segales, Joaquim ;
Mateu, Enric .
VETERINARY MICROBIOLOGY, 2007, 125 (3-4) :244-255
[7]   Virus-like particles: Passport to immune recognition [J].
Grgacic, Elizabeth V. L. ;
Anderson, David A. .
METHODS, 2006, 40 (01) :60-65
[8]   COMPARISON OF THE PATHOGENICITY OF 2 US PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS ISOLATES WITH THAT OF THE LELYSTAD VIRUS [J].
HALBUR, PG ;
PAUL, PS ;
FREY, ML ;
LANDGRAF, J ;
EERNISSE, K ;
MENG, XJ ;
LUM, MA ;
ANDREWS, JJ ;
RATHJE, JA .
VETERINARY PATHOLOGY, 1995, 32 (06) :648-660
[9]   Evaluation of the Efficacy and Cross-Protective Immunity of Live-Attenuated Chimeric PCV1-2b Vaccine Against PCV2b and PCV2d Subtype Challenge in Pigs [J].
Huan, Changchao ;
Fan, Mingyu ;
Cheng, Qingru ;
Wang, Xiaobo ;
Gao, Qingqing ;
Wang, Wanbin ;
Gao, Song ;
Liu, Xiufan .
FRONTIERS IN MICROBIOLOGY, 2018, 9
[10]   Genetic diversity and different cross-neutralization capability of porcine circovirus type 2 isolates recently circulating in South Korea [J].
Kang, Seok-Jin ;
Kang, Hyeonjeong ;
You, Su-Hwa ;
Lee, Hye Jeong ;
Lee, Nakhyung ;
Hyun, Bang-Hun ;
Cha, Sang-Ho .
BMC VETERINARY RESEARCH, 2020, 16 (01)