Selective incorporation of 19F-labeled Trp side chains for NMR-spectroscopy-based ligand-protein interaction studies

被引：26

作者：

Leone, M ^{[1
]}

Rodriguez-Mias, RA ^{[1
]}

Pellecchia, M ^{[1
]}

机构：

[1] Burnham Inst, San Diego, CA 92103 USA

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 07期

关键词：

drug design; isotopic labeling; NMR spectroscopy; protein-ligand interactions;

D O I：

10.1002/cbic.200300597

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

F marks the spot: We report a new technique for selectively labeling Trp side chains in proteins with 19F by using 3-β-indoleacrilic acid as an inhibitor of tryptophan biosynthesis. Our method finds useful application in the study of protein-protein or protein - ligand interactions (see figure) by means of 19F NMR spectroscopy.

引用

页码：649 / 650

页数：2

共 12 条

[11] Slow exchange in the chromophore of a green fluorescent protein variant [J].