Exposure to perfluorinated compounds: in vitro study on thyroid cells

被引：51

作者：

Coperchini, Francesca ^{[1
,2
]}

Pignatti, Patrizia ^{[3
]}

Lacerenza, Serena ^{[1
,2
]}

Negri, Sara ^{[4
]}

Sideri, Riccardo ^{[1
,2
]}

Testoni, Claudia ^{[3
]}

de Martinis, Luca ^{[1
,2
]}

Cottica, Danilo ^{[4
]}

Magri, Flavia ^{[1
,2
]}

Imbriani, Marcello ^{[5
]}

Rotondi, Mario ^{[1
,2
]}

Chiovato, Luca ^{[1
,2
]}

机构：

[1] Univ Pavia, Fdn Salvatore Maugeri IRCCS, Unit Internal Med & Endocrinol, Lab Endocrine Disruptors, I-27100 Pavia, Italy

[2] Univ Pavia, Chair Endocrinol, I-27100 Pavia, Italy

[3] Fdn Salvatore Maugeri IRCCS, Unit Allergy & Immunol Unit, Pavia, Italy

[4] Fdn Salvatore Maugeri IRCCS, Environm Res Ctr, Pavia, Italy

[5] Salvatore Maugeri Fdn, Environm & Occupat Med Unit, Pavia, Italy

来源：

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 2015年 / 22卷 / 03期

关键词：

Perfluorinated chemicals (PFCs); Perfluorooctanoic acid (PFOA); Perfluorooctane sulfonate (PFOS); Fisher rat thyroid line-5 (FRTL-5); Cytotoxicity; PERFLUOROOCTANOIC ACID; FATTY-ACIDS; SULFONATE; TOXICITY; PFCS; PFOS; CYTOTOXICITY; CHEMICALS; BINDING; HEALTH;

D O I：

10.1007/s11356-014-3480-9

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are widely used perfluorinated chemicals (PFCs). Previous studies detected PFOA and PFOS in human tissues including the thyroid gland. There are no studies on the in vitro effects of PFOA and PFOS on thyroid cells. Our study was aimed at evaluating the effect of the in vitro exposure to PFOA and PFOS on thyroid cell proliferation and viability. These objectives were investigated using Fisher rat thyroid line-5 (FRTL-5) cells. FRTL-5 cells cultured in the presence of PFOA and PFOS at concentrations up to 10(4) nM do not display changes in their viability and proliferation rate, while at a concentration of 10(5) nM of either PFCs, a significant inhibition of cell proliferation, mainly due to increased cell death, was found. PFOA and PFOS were detected in FRTL-5 cell pellets after 72 h of incubation with PFCs but not in control cultures. When FRTL-5 were incubated with PFCs then washed in PBS and re-cultured for 72 h without PFCs in the medium, no detectable concentrations of PFOA and PFOS were measured in the cell pellet. This indicates that PFOA and PFOS enter thyroid cells by a gradient-based passive diffusion mechanism. Future studies are required to evaluate the potential toxic effect resulting from prolonged in vivo exposure to even lower concentrations of PFCs.

引用

页码：2287 / 2294

页数：8

共 40 条

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