Homology-based fold predictions for Mycoplasma genitalium proteins

被引：90

作者：

Huynen, M

Doerks, T

Eisenhaber, F

Orengo, C

Sunyaev, S

Yuan, YP

Bork, P

机构：

[1] European Mol Biol Lab, D-69012 Heidelberg, Germany

[2] Max Delbruck Ctr Mol Med, Berlin, Germany

[3] UCL, Dept Biochem & Mol Biol, London, England

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1998年 / 280卷 / 03期

关键词：

structure prediction; genome analysis; Mycoplasma genitalium; homology modeling; fold assignment;

D O I：

10.1006/jmbi.1998.1884

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Homology search techniques based on the iterative PSI-BLAST method in combination with various filters for low sequence complexity are applied to assign folds to all Mycoplasma genitalium proteins. The resulting procedure (implemented as a web server) is able to predict at least one domain in 37% of these proteins automatically, with an estimated accuracy higher than 98%. Taking structural features such as coiled coil or transmembrane regions aside, folds can be assigned to more than half of the globular proteins in a bacterium just by iterative sequence comparison. (C) 1998 Academic Press.

引用

页码：323 / 326

页数：4

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