Normal regulation of Rbf1/E2f1 target genes in Drosophila type 1 protein phosphatase mutants

被引：14
作者：
Swanhart, Lisa M.
Sanders, Akeisha N.
Duronio, Robert J.
机构：
[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Program Mol Biol & Biotechnol, Chapel Hill, NC 27599 USA
[3] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA
[4] Univ N Carolina, Res Educ Support Program, Chapel Hill, NC 27599 USA
来源：
DEVELOPMENTAL DYNAMICS | 2007年 / 236卷 / 09期
关键词：
Drosophila; pRb; PP1; cell cycle;
D O I：
10.1002/dvdy.21265
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
G1 CycIin/Cdk complexes phosphorylate and inactivate the pRb tumor suppressor by preventing its ability to bind and repress E2F transcription factors. Current molecular and biochemical evidence suggests that type 1 protein phosphatases (PP1) dephosphorylate and thereby activate pRb, but the functional significance of this has not been addressed in the context of animal development. Here, we use genetic analyses to determine the role of PP1 in the regulation of Rbf1 activity during Drosophila development. While Rbf1 is required for E2f1 inhibition and G1 arrest in the embryonic epidermis and for the periodic expression of E2f1 target genes during endocycle S phase in the embryonic midgut and larval salivary gland, PP1 is not. PP1 regulates periodic cyclin E protein accumulation in ovarian nurse cells independently of Rbf1, which is dispensable for endocycle regulation in this tissue. We conclude that PP1 is not a major regulator of the Rbf1/E2F1 pathway in Drosophila.
引用
收藏
页码：2567 / 2577
页数：11
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