Celastrol inhibits colorectal cancer through TGF-β1/Smad signaling

被引：39
作者：
Jiang, Zhitao [1 ]
Cao, Qianyu [2 ]
Dai, Guoliang [3 ]
Wang, Jianchun [1 ]
Liu, Chundi [1 ]
Lv, Lingyan [1 ]
Pan, Jinhuo [4 ]
机构：
[1] Nanjing Univ Chinese Med, Dept Pharm Off, Zhangjiagang Hosp Tradit Chinese Med, Zhangjiagang, Peoples R China
[2] Nanjing Univ Chinese Med, First Clin Coll, Nanjing, Jiangsu, Peoples R China
[3] Nanjing Univ Chinese Med, Affiliated Hosp, Dept Clin Pharmacol, Nanjing, Jiangsu, Peoples R China
[4] Nanjing Univ Chinese Med, Coll Pharm, 138 Xianlin Rd, Nanjing 210038, Jiangsu, Peoples R China
来源：
ONCOTARGETS AND THERAPY | 2019年 / 12卷
关键词：
celastrol; colorectal cancer; TGF-beta; 1; Smad; TGF-BETA; INFLAMMATORY CYTOKINES; OXALIPLATIN; ADHESION; COLON; CELLS;
D O I：
10.2147/OTT.S187817
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background: There are few clinical challenges associated with the treatment of colorectal cancer (CRC). Studies have shown that TGF-beta plays a crucial role in CRC. Importantly, celastrol, a major components of the root extract of the traditional Chinese herb Tripterygium wilfordii Hook F, has been shown to inhibit the growth, adhesion, and metastasis of human CRC cells through the inhibition of TGF-beta 1/Smad signaling. Materials and methods: Real-time PCR and Western blot tests were proceeded to present TGF-beta 1, TGF-beta receptor type I (TGF beta RI), TGF-beta receptor type II (TGF beta RII), Smad2/3, p-Smad2/3, Smad4, and glyceraldehyde-3-phosphate dehydrogenase expression in human colon cancer cell samples. Results: Our results indicated that celastrol can reduce the expression levels of TGF-beta 1, TGF beta RI, and TGF beta RII in HCT116 and SW620 cells. Furthermore, celastrol could also prevent the increase in Smad4 and p-Smad2/3 in HCT116 and SW620 cells. Conclusion: Celastrol could inhibit tumor growth through TGF-beta 1/Smad signaling and might be a promising therapeutic component against CRC.
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收藏
页码：509 / 518
页数：10
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