Electrochemical genomagnetic assay for the detection of hepatitis B virus DNA in polymerase chain reaction amplicons by using disposable sensor technology

被引：78

作者：

Erdem, A ^{[1
]}

Ariksoysal, DO

Karadeniz, H

Kara, P

Sengonul, A

Sayiner, AA

Ozsoz, M

机构：

[1] Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey

[2] Dokuz Eylul Univ, Sch Med, Dept Microbiol, Izmir, Turkey

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2005年 / 7卷 / 08期

关键词：

magnetic particles; DNA hybridization; pencil graphite electrode; electrochemical genosensor; guanine oxidation signal; hepatitis B wild type virus;

D O I：

10.1016/j.elecom.2005.05.006

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A genomagnetic assay coupling of electrochemical monitoring for the detection of wild type hepatitis B virus (HBV) DNA in polymerase chain reaction (PCR) amplicons has been described. The development of this technology combined with a disposable sensor, pencil graphite electrode (PGE) and differential pulse voltammetry (DPV) was performed by using 20-mer synthetic oligonucleotides, and PCR amplicons in length 437-bp as measuring the guanine oxidation signal observed at +1.0 V after DNA hybridization with HBV probe. The detection limit estimated from S/N= 3, corresponds to 74.8 fmole/mL target concentration in the 50 mu L samples. The characterization, optimization and advantages of the genomagnetic assay are discussed with its detection limit and reproducibility in comparison with previous electrochemical assays for DNA hybridization. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：815 / 820

页数：6

共 34 条

[1] Covalently bonded adducts of deoxyribonucleic acid (DNA) oligonucleotides with single-wall carbon nanotubes: Synthesis and hybridization [J].

Baker, SE ;

Cai, W ;

Lasseter, TL ;

Weidkamp, KP ;

Hamers, RJ .

NANO LETTERS, 2002, 2 (12) :1413-1417

[2] Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus [J].

Cane, PA ;

Cook, P ;

Ratcliffe, D ;

Mutimer, D ;

Pillay, D .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) :1600-1608

[3] An immunomagnetic electrochemical sensor based an a perfluorosulfonate-coated screen-printed electrode for the determination of 2,4-dichlorophenoxyacetic acid [J].

Dequaire, M ;

Degrand, C ;

Limoges, B .

ANALYTICAL CHEMISTRY, 1999, 71 (13) :2571-2577

[4]

*EASL JUR, 2003, J HEPATOL, V38, P533

[5] Novel hybridization indicator methylene blue for the electrochemical detection of short DNA sequences related to the hepatitis B virus [J].

Erdem, A ;

Kerman, K ;

Meric, B ;

Akarca, US ;

Ozsoz, M .

ANALYTICA CHIMICA ACTA, 2000, 422 (02) :139-149

[6] Rigid carbon composites: a new transducing material for label-free electrochemical genosensing [J].

Erdem, A ;

Pividori, MI ;

del Valle, M ;

Alegret, S .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2004, 567 (01) :29-37

[7]

Erdem A, 2002, ELECTROANAL, V14, P965, DOI 10.1002/1521-4109(200208)14:14<965::AID-ELAN965>3.0.CO

[8]

2-U

[9]

Erdem A, 1999, ELECTROANAL, V11, P586

[10] Two-surface strategy in electrochemical DNA hybridization assays: Detection of osmium-labeled target DNA at carbon electrodes [J].

Fojta, M ;

Havran, L ;

Billova, S ;

Kostecka, P ;

Masarik, M ;

Kizek, R .

ELECTROANALYSIS, 2003, 15 (5-6) :431-440

← 1 2 3 4 →