Comparative genomics identifies the Magnaporthe oryzae avirulence effector AvrPi9 that triggers Pi9-mediated blast resistance in rice

被引：195

作者：

Wu, Jun ^{[1
,2
]}

Kou, Yanjun ^{[3
]}

Bao, Jiandong ^{[2
,4
]}

Li, Ya ^{[4
]}

Tang, Mingzhi ^{[2
]}

Zhu, Xiaoli ^{[2
,5
]}

Ponaya, Ariane ^{[5
,6
]}

Xiao, Gui ^{[5
]}

Li, Jinbin ^{[7
]}

Li, Chenyun ^{[8
]}

Song, Min-Young ^{[9
]}

Cumagun, Christian Joseph R. ^{[6
]}

Deng, Qiyun ^{[1
]}

Lu, Guodong ^{[4
]}

Jeon, Jong-Seong ^{[9
]}

Naqvi, Naweed I. ^{[3
]}

Zhou, Bo ^{[2
,5
]}

机构：

[1] Cent South Univ, Grad Sch, State Key Lab Hybrid Rice, Longping Branch, Changsha 410125, Hunan, Peoples R China

[2] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

[3] Natl Univ Singapore, Dept Biol Sci, Temasek Life Sci Lab, Singapore 117548, Singapore

[4] Fujian Agr & Forestry Univ, Key Lab Biopesticide & Chem Biol, Minist Educ, Fuzhou 350002, Peoples R China

[5] Int Rice Res Inst, Manila 1301, Philippines

[6] Univ Philippines, Coll Agr, Los Banos 4031, Laguna, Philippines

[7] Yunnan Acad Agr Sci, Agr Environm & Resources Res Inst, Kunming 650201, Peoples R China

[8] Yunnan Agr Univ, Minist China, Key Lab Agrobiodivers & Pest Management Educ, Kunming 650201, Yunnan, Peoples R China

[9] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea

来源：

NEW PHYTOLOGIST | 2015年 / 206卷 / 04期

基金：

中国国家自然科学基金; 新加坡国家研究基金会;

关键词：

AvrPi9; Magnaporthe oryzae; resistance genes; rice; specificity; ARMS-RACE; GENE; FUNGUS; PROTEIN; EVOLUTION; DYNAMICS; LOCUS; SPECIFICITY; COEVOLUTION; SEQUENCE;

D O I：

10.1111/nph.13310

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We identified the Magnaporthe oryzae avirulence effector AvrPi9 cognate to rice blast resistance gene Pi9 by comparative genomics of requisite strains derived from a sequential planting method.AvrPi9 encodes a small secreted protein that appears to localize in the biotrophic interfacial complex and is translocated to the host cell during rice infection. AvrPi9 forms a tandem gene array with its paralogue proximal to centromeric region of chromosome 7. AvrPi9 is expressed highly at early stages during initiation of blast disease. Virulent isolate strains contain Mg-SINE within the AvrPi9 coding sequence. Loss of AvrPi9 did not lead to any discernible defects during growth or pathogenesis in M.oryzae. This study reiterates the role of diverse transposable elements as off-switch agents in acquisition of gain-of-virulence in the rice blast fungus. The prevalence of AvrPi9 correlates well with the avirulence pathotype in diverse blast isolates from the Philippines and China, thus supporting the broad-spectrum resistance conferred by Pi9 in different rice growing areas. Our results revealed that Pi9 and Piz-t at the Pi2/9 locus activate race specific resistance by recognizing sequence-unrelated AvrPi9 and AvrPiz-t genes, respectively.

引用

页码：1463 / 1475

页数：13

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