Association of Cytokines With Exosomes in the Plasma of HIV-1-Seropositive Individuals

被引：77

作者：

Konadu, Kateena Addae ^{[1
]}

Chu, Jane ^{[1
]}

Huang, Ming Bo ^{[1
]}

Amancha, Praveen Kumar ^{[2
,3
]}

Armstrong, Wendy ^{[4
]}

Powell, Michael D. ^{[1
]}

Villinger, Francois ^{[2
,3
]}

Bond, Vincent C. ^{[1
]}

机构：

[1] Emory Univ, Sch Med, Morehouse Sch Med, Dept Microbiol Biochem & Immunol, Atlanta, GA USA

[2] Emory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA

[3] Emory Univ, Sch Med, Yerkes Natl Primate Res Ctr, Atlanta, GA 30322 USA

[4] Emory Univ, Sch Med, Dept Med, Atlanta, GA 30322 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2015年 / 211卷 / 11期

基金：

美国国家卫生研究院;

关键词：

exosomes; HIV; cytokines; chemokines; immune activation; HIV TYPE-1 NEF; T-CELLS; INFECTED-CELLS; MICROVESICLES; VESICLES;

D O I：

10.1093/infdis/jiu676

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human immunodeficiency virus (HIV)-infected and viremic individuals exhibit elevated levels of plasma cytokines. Here we show that most cytokines are not in free form but appear associated with exosomes that are distinct from virions. Purified exosomes were analyzed to determine the levels of 21 cytokines and chemokines and compared with exosome-depleted plasma. Most cytokines were markedly enriched in exosomes from HIV-positive individuals relative to negative controls and to plasma. Moreover, exposure of naive peripheral blood mononuclear cells to exosomes purified from HIV-positive patients induced CD38 expression on naive and central memory CD4(+) and CD8(+) T cells, probably contributing to inflammation and viral propagation via bystander cell activation.

引用

页码：1712 / 1716

页数：5

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