Identification of genetic loci for flag-leaf-related traits in wheat (Triticum aestivum L.) and their effects on grain yield

被引：7

作者：

Wang, Ying ^{[1
,2
]}

Qiao, Ling ^{[1
]}

Yang, Chenkang ^{[1
,2
]}

Li, Xiaohua ^{[1
]}

Zhao, Jiajia ^{[1
]}

Wu, Bangbang ^{[1
]}

Zheng, Xingwei ^{[1
]}

Li, Pengbo ^{[3
]}

Zheng, Jun ^{[1
]}

机构：

[1] Shanxi Agr Univ, Inst Wheat Res, Linfen, Peoples R China

[2] Shanxi Univ, Sch Life Sci, Taiyuan, Peoples R China

[3] Shanxi Agr Univ, Inst Cotton Res, Yuncheng, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷

关键词：

wheat; flag leaf; flag leaf volume measurement; quantitative trait loci; grain; WINTER-WHEAT; QTL; SENESCENCE; NITROGEN; PHOTOSYNTHESIS; MORPHOLOGY; RATES; ANGLE; AREA;

D O I：

10.3389/fpls.2022.990287

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Flag-leaf-related traits including length (FLL), width (FLW), area (FLA), thickness (FLT), and volume (FLV) of flag leaves are the most important determinants of plant architecture and yield in wheat. Understanding the genetic basis of these traits could accelerate the breeding of high yield wheat varieties. In this study, we constructed a doubled haploid (DH) population and analyzed flag-leaf-related traits in five experimental locations/years using the wheat 90K single-nucleotide polymorphism array. It's worth noting that a novel method was used to measure FLT and FLV easily. Leaf thickness at two-thirds of the leaf length from tip to collar represented the average leaf thickness as measured with freehand sections and was used to calculate the leaf volume. In addition, flag-leaf-related traits showed positive correlations with yield related traits under two different water regimes. A total of 79 quantitative trait loci (QTL) controlling the five traits were detected among all chromosomes except 4D and 5A, explaining 3.09-14.52% of the phenotypic variation. Among them, 15 stable QTL were identified in more than three environments, including two major QTL for FLT, six for FLW, three for FLA, two for FLT and two for FLV. DH lines with positive alleles at both QTL regions had an average FLL (9.90%), FLW (32.87%), FLT (6.62%), FLA (18.47%), and FLV (20.87%) greater than lines with contrasting alleles. QFLT-2B, QFLV-2A, and QFLV-7D were co-located with yield-related traits. The 15 QTL were validated by tightly linked kompetitive allele specific PCR (KASP) markers in a recombinant inbred line (RIL) population derived from a different cross. QFLL-4A, QFLW-4B, QFLA-5D.1, QFLA-7A, QFLA-7D.1, QFLT-2B, QFLT-6A, QFLV-2A, and QFLV-7D are likely novel loci. These results provide a better understanding of the genetic basis underlying flag-leaf-related traits. Also, target regions for fine mapping and marker-assisted selection were identified and these will be valuable for breeding high yielding bread wheat.

引用

页数：17

共 54 条

[41] Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature [J].

Vijayalakshmi, Kolluru ;

Fritz, Allan K. ;

Paulsen, Gary M. ;

Bai, Guihua ;

Pandravada, Satchidanand ;

Gill, Bikram S. .

MOLECULAR BREEDING, 2010, 26 (02) :163-175

[42] Identifying Loci Influencing 1,000-Kernel Weight in Wheat by Microsatellite Screening for Evidence of Selection during Breeding [J].

Wang, Lanfen ;

Ge, Hongmei ;

Hao, Chenyang ;

Dong, Yushen ;

Zhang, Xueyong .

PLOS ONE, 2012, 7 (02)

[43] QTL mapping for grain filling rate and yield-related traits in RILs of the Chinese winter wheat population Heshangmai X Yu8679 [J].

Wang, R. X. ;

Hai, L. ;

Zhang, X. Y. ;

You, G. X. ;

Yan, C. S. ;

Xiao, S. H. .

THEORETICAL AND APPLIED GENETICS, 2009, 118 (02) :313-325

[44] Anatomical and Physiological Plasticity in Leymus chinensis (Poaceae) along Large-Scale Longitudinal Gradient in Northeast China [J].

Wang, Renzhong ;

Huang, Wenwen ;

Chen, Liang ;

Ma, Linna ;

Guo, Chengyuan ;

Liu, Xiaoqiang .

PLOS ONE, 2011, 6 (11)

[45] Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array [J].

Wang, Shichen ;

Wong, Debbie ;

Forrest, Kerrie ;

Allen, Alexandra ;

Chao, Shiaoman ;

Huang, Bevan E. ;

Maccaferri, Marco ;

Salvi, Silvio ;

Milner, Sara G. ;

Cattivelli, Luigi ;

Mastrangelo, Anna M. ;

Whan, Alex ;

Stephen, Stuart ;

Barker, Gary ;

Wieseke, Ralf ;

Plieske, Joerg ;

Lillemo, Morten ;

Mather, Diane ;

Appels, Rudi ;

Dolferus, Rudy ;

Brown-Guedira, Gina ;

Korol, Abraham ;

Akhunova, Alina R. ;

Feuillet, Catherine ;

Salse, Jerome ;

Morgante, Michele ;

Pozniak, Curtis ;

Luo, Ming-Cheng ;

Dvorak, Jan ;

Morell, Matthew ;

Dubcovsky, Jorge ;

Ganal, Martin ;

Tuberosa, Roberto ;

Lawley, Cindy ;

Mikoulitch, Ivan ;

Cavanagh, Colin ;

Edwards, Keith J. ;

Hayden, Matthew ;

Akhunov, Eduard .

PLANT BIOTECHNOLOGY JOURNAL, 2014, 12 (06) :787-796

[46] QTL mapping of flag leaf traits in common wheat using an integrated high-density SSR and SNP genetic linkage map [J].

Wu, Qiuhong ;

Chen, Yongxing ;

Fu, Lin ;

Zhou, Shenghui ;

Chen, Jiaojiao ;

Zhao, Xiaojie ;

Zhang, Dong ;

Ouyang, Shuhong ;

Wang, Zhenzhong ;

Li, Dan ;

Wang, Guoxin ;

Zhang, Deyun ;

Yuan, Chengguo ;

Wang, Lixin ;

You, Mingshan ;

Han, Jun ;

Liu, Zhiyong .

EUPHYTICA, 2016, 208 (02) :337-351

[47]

Xu HengYong Xu HengYong, 1995, Acta Agronomica Sinica, V21, P204

[48] Fine mapping TaFLW1, a major QTL controlling flag leaf width in bread wheat (Triticum aestivum L.) [J].

Xue, Shulin ;

Xu, Feng ;

Li, Guoqiang ;

Zhou, Yan ;

Lin, Musen ;

Gao, Zhongxia ;

Su, Xiuhong ;

Xu, Xiaowu ;

Jiang, Ge ;

Zhang, Shuang ;

Jia, Haiyan ;

Kong, Zhongxin ;

Zhang, Lixia ;

Ma, Zhengqiang .

THEORETICAL AND APPLIED GENETICS, 2013, 126 (08) :1941-1949

[49] Identification of genetic loci and a candidate gene related to flag leaf traits in common wheat by genome-wide association study and linkage mapping [J].

Yan, Xuefang ;

Zhao, Lei ;

Ren, Yan ;

Zhang, Ning ;

Dong, Zhongdong ;

Chen, Feng .

MOLECULAR BREEDING, 2020, 40 (06)

[50] Identification of Genetic Loci Affecting Flag Leaf Chlorophyll in Wheat Grown under Different Water Regimes [J].

Yang, Bin ;

Wen, Xiaojie ;

Wen, Hongwei ;

Feng, Yanru ;

Zhao, Jiajia ;

Wu, Bangbang ;

Zheng, Xingwei ;

Yang, Chenkang ;

Yang, Sanwei ;

Qiao, Ling ;

Zheng, Jun .

FRONTIERS IN GENETICS, 2022, 13

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