Identification of genetic loci for flag-leaf-related traits in wheat (Triticum aestivum L.) and their effects on grain yield

被引：7

作者：

Wang, Ying ^{[1
,2
]}

Qiao, Ling ^{[1
]}

Yang, Chenkang ^{[1
,2
]}

Li, Xiaohua ^{[1
]}

Zhao, Jiajia ^{[1
]}

Wu, Bangbang ^{[1
]}

Zheng, Xingwei ^{[1
]}

Li, Pengbo ^{[3
]}

Zheng, Jun ^{[1
]}

机构：

[1] Shanxi Agr Univ, Inst Wheat Res, Linfen, Peoples R China

[2] Shanxi Univ, Sch Life Sci, Taiyuan, Peoples R China

[3] Shanxi Agr Univ, Inst Cotton Res, Yuncheng, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷

关键词：

wheat; flag leaf; flag leaf volume measurement; quantitative trait loci; grain; WINTER-WHEAT; QTL; SENESCENCE; NITROGEN; PHOTOSYNTHESIS; MORPHOLOGY; RATES; ANGLE; AREA;

D O I：

10.3389/fpls.2022.990287

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Flag-leaf-related traits including length (FLL), width (FLW), area (FLA), thickness (FLT), and volume (FLV) of flag leaves are the most important determinants of plant architecture and yield in wheat. Understanding the genetic basis of these traits could accelerate the breeding of high yield wheat varieties. In this study, we constructed a doubled haploid (DH) population and analyzed flag-leaf-related traits in five experimental locations/years using the wheat 90K single-nucleotide polymorphism array. It's worth noting that a novel method was used to measure FLT and FLV easily. Leaf thickness at two-thirds of the leaf length from tip to collar represented the average leaf thickness as measured with freehand sections and was used to calculate the leaf volume. In addition, flag-leaf-related traits showed positive correlations with yield related traits under two different water regimes. A total of 79 quantitative trait loci (QTL) controlling the five traits were detected among all chromosomes except 4D and 5A, explaining 3.09-14.52% of the phenotypic variation. Among them, 15 stable QTL were identified in more than three environments, including two major QTL for FLT, six for FLW, three for FLA, two for FLT and two for FLV. DH lines with positive alleles at both QTL regions had an average FLL (9.90%), FLW (32.87%), FLT (6.62%), FLA (18.47%), and FLV (20.87%) greater than lines with contrasting alleles. QFLT-2B, QFLV-2A, and QFLV-7D were co-located with yield-related traits. The 15 QTL were validated by tightly linked kompetitive allele specific PCR (KASP) markers in a recombinant inbred line (RIL) population derived from a different cross. QFLL-4A, QFLW-4B, QFLA-5D.1, QFLA-7A, QFLA-7D.1, QFLT-2B, QFLT-6A, QFLV-2A, and QFLV-7D are likely novel loci. These results provide a better understanding of the genetic basis underlying flag-leaf-related traits. Also, target regions for fine mapping and marker-assisted selection were identified and these will be valuable for breeding high yielding bread wheat.

引用

页数：17

共 54 条

[31] Eliminating the purple acid phosphatase AtPAP26 in Arabidopsis thaliana delays leaf senescence and impairs phosphorus remobilization [J].

Robinson, Whitney D. ;

Carson, Ira ;

Ying, Sheng ;

Ellis, Kaya ;

Plaxton, William C. .

NEW PHYTOLOGIST, 2012, 196 (04) :1024-1029

[32] Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice [J].

Sakamoto, T ;

Morinaka, Y ;

Ohnishi, T ;

Sunohara, H ;

Fujioka, S ;

Ueguchi-Tanaka, M ;

Mizutani, M ;

Sakata, K ;

Takatsuto, S ;

Yoshida, S ;

Tanaka, H ;

Kitano, H ;

Matsuoka, M .

NATURE BIOTECHNOLOGY, 2006, 24 (01) :105-109

[33] The genetic control of flag leaf length in normal and late sown durum wheat [J].

Sharma, SN ;

Sain, RS ;

Sharma, RK .

JOURNAL OF AGRICULTURAL SCIENCE, 2003, 141 :323-331

[34] Inheritance of flag-leaf angle, flag-leaf area and flag leaf area duration in four wheat crosses [J].

Simón, MR .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (02) :310-314

[35] Evaluation of simple methods for estimating broad-sense heritability in stands of randomly planted genotypes [J].

Smith, SE ;

Kuehl, RO ;

Ray, IM ;

Hui, R ;

Soleri, D .

CROP SCIENCE, 1998, 38 (05) :1125-1129

[36] Regulation of Leaf Morphology by MicroRNA394 and its Target LEAF CURLING RESPONSIVENESS [J].

Song, Jian Bo ;

Huang, Si Qi ;

Dalmay, Tamas ;

Yang, Zhi Min .

PLANT AND CELL PHYSIOLOGY, 2012, 53 (07) :1283-1294

[37] Canopy structure, light interception, and photosynthesis in maize [J].

Stewart, DW ;

Costa, C ;

Dwyer, LM ;

Smith, DL ;

Hamilton, RI ;

Ma, BL .

AGRONOMY JOURNAL, 2003, 95 (06) :1465-1474

[38] ON THE RELATIONSHIP BETWEEN LEAF ANATOMY AND CO2 DIFFUSION THROUGH THE MESOPHYLL OF HYPOSTOMATOUS LEAVES [J].

SYVERTSEN, JP ;

LLOYD, J ;

MCCONCHIE, C ;

KRIEDEMANN, PE ;

FARQUHAR, GD .

PLANT CELL AND ENVIRONMENT, 1995, 18 (02) :149-157

[39] A Quantitative Trait Locus for Chlorophyll Content and its Association with Leaf Photosynthesis in Rice [J].

Takai, Toshiyuki ;

Kondo, Motohiko ;

Yano, Masahiro ;

Yamamoto, Toshio .

RICE, 2010, 3 (2-3) :172-180

[40] QTL mapping and validation of bread wheat flag leaf morphology across multiple environments in different genetic backgrounds [J].

Tu, Yang ;

Liu, Hang ;

Liu, Jiajun ;

Tang, Huaping ;

Mu, Yang ;

Deng, Mei ;

Jiang, Qiantao ;

Liu, Yaxi ;

Chen, Guoyue ;

Wang, Jirui ;

Qi, Pengfei ;

Pu, Zhien ;

Chen, Guangdeng ;

Peng, Yuanying ;

Jiang, Yunfeng ;

Xu, Qiang ;

Kang, Houyang ;

Lan, Xiujin ;

Wei, Yuming ;

Zheng, Youliang ;

Ma, Jian .

THEORETICAL AND APPLIED GENETICS, 2021, 134 (01) :261-278

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