Thermally assisted hydrolysis and methylation of humic fractions obtained by polyacrylamide gel electrophoresis

被引：6

作者：

Hermosin, B

Trubetskoj, OA

Trubetskaya, OE

Saiz-Jimenez, C

机构：

[1] CSIC, Inst Recursos Nat & Agrobiol, E-41080 Seville, Spain

[2] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142292, Moscow Region, Russia

[3] Russian Acad Sci, Branch Shemyakin Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia

来源：

JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS | 2001年 / 58卷

关键词：

humic acid; pyrolysis; polyacrylamide gel electrophoresis; microbial fatty acid;

D O I：

10.1016/S0165-2370(00)00213-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper we applied thermally assisted hydrolysis and methylation to a soil humic acid (HA) and their polyacrylamide gel electrophoresis (PAGE) fractions. The data indicates that PAGE fractionation of HA produces selectively enriched humic fractions in terms of major structural components (e.g. fatty acids, lignin units, etc.). Lignin components were mainly found in fraction b (medium electrophoretic mobility) of HA. Plant fatty acids were mainly distributed in fraction a (no electrophoretic mobility), and microbial lipids were distributed in all fractions, but the higher relative amounts appeared primarily associated with fractions b and c + d (high electrophoreticmobility). (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：341 / 347

页数：7

共 12 条

[1]

De Nobili M., 1989, HUMIC SUBSTANCES, P561

[2]

Duxbury JM, 1989, HUMIC SUBSTANCES, P593

[3] Characterization of the tetramethylammonium hydroxide thermochemolysis products of carbohydrates [J].