Zn2+-Dependent peptide nucleic acid-based artificial ribonucleases with unprecedented efficiency and specificity

被引：9

作者：

Luige, Olivia ^{[1
]}

Bose, Partha Pratim ^{[1
]}

Stulz, Rouven ^{[1
,2
,3
]}

Steunenberg, Peter ^{[1
,4
]}

Brun, Omar ^{[1
,5
]}

Andersson, Shalini ^{[2
]}

Murtola, Merita ^{[1
]}

Stromberg, Roger ^{[1
]}

机构：

[1] Karolinska Inst, Dept Biosci & Nutr, Neo, S-14183 Huddinge, Sweden

[2] AstraZeneca, BioPharmaceut R&D, Discovery Sci, Oligonucleotide Discovery, Gothenburg, Sweden

[3] AstraZeneca, BioPharmaceut R&D, Res & Early Dev Cardiovasc Renal & Metab, DMPK, Gothenburg, Sweden

[4] TNO Delft, Leeghwaterstr 44, NL-2628 CA Delft, Netherlands

[5] Carrer Can Vinyalets 11, Sta Perpetua de Mogoda 08130, Spain

来源：

CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 83期

关键词：

PHOSPHODIESTER BONDS; RNA BULGES; CLEAVAGE; ZINC; ION; MECHANISM; DNA;

D O I：

10.1039/d1cc04383h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present Zn2+-dependent dimethyl-dipyridophenazine PNA conjugates as efficient RNA cleaving artificial enzymes. These PNAzymes display site-specific RNA cleavage with 10 minute half-lives and cleave clinically relevant RNA models.

引用

页码：10911 / 10914

页数：4

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