In this paper, the frequency of the microaerophilic gram-negative bacterium Actinobacillus actinomycetemcomitans (Ac) which is a component of the normal human oral microflora was investigated. Ac could be cultivated from oral material (molar sulcus and/or mucous membrane of the cheek) from 55 out of 405 healthy adults examined. This overall Ac frequency of 13.6% corresponds well to the few other reports existing in literature. A short discussion about the possible pathogenic potential of Ac is given at the end of the paper.