JARID2 Is Involved in Transforming Growth Factor-Beta-Induced Epithelial-Mesenchymal Transition of Lung and Colon Cancer Cell Lines

被引：60
作者：
Tange, Shoichiro [1 ]
Oktyabri, Dulamsuren [1 ]
Terashima, Minoru [1 ]
Ishimura, Akihiko [1 ]
Suzuki, Takeshi [1 ]
机构：
[1] Kanazawa Univ, Canc Res Inst, Div Funct Genom, Kanazawa, Ishikawa 920, Japan
来源：
PLOS ONE | 2014年 / 9卷 / 12期
关键词：
GROUP PROTEIN EZH2; GENE-EXPRESSION; MIR-200; FAMILY; STEM-CELLS; E-CADHERIN; HISTONE; PRC2; PROGRESSION; SNAIL; METHYLATION;
D O I：
10.1371/journal.pone.0115684
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Histone methylation plays a crucial role in various biological and pathological processes including cancer development. In this study, we discovered that JARID2, an interacting component of Polycomb repressive complex-2 (PRC2) that catalyzes methylation of lysine 27 of histone H3 (H3K27), was involved in Transforming Growth Factor-beta (TGF-beta)-induced epithelial-mesenchymal transition (EMT) of A549 lung cancer cell line and HT29 colon cancer cell line. The expression of JARID2 was increased during TGF-beta-induced EMT of these cell lines and knockdown of JARID2 inhibited TGF-beta-induced morphological conversion of the cells associated with EMT. JARID2 knockdown itself had no effect in the expression of EMT-related genes but antagonized TGF-beta-dependent expression changes of EMT-related genes such as CDH1, ZEB family and microRNA-200 family. Chromatin immunoprecipitation assays showed that JARID2 was implicated in TGF-beta-induced transcriptional repression of CDH1 and microRNA-200 family genes through the regulation of histone H3 methylation and EZH2 occupancies on their regulatory regions. Our study demonstrated a novel role of JARID2 protein, which may control PRC2 recruitment and histone methylation during TGF-beta-induced EMT of lung and colon cancer cell lines.
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页数：25
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共 36 条
[11]   The JARID2-PRC2 duality [J].
Herz, Hans-Martin ;
Shilatifard, Ali .
GENES & DEVELOPMENT, 2010, 24 (09) :857-861
[12]   The basics of epithelial-mesenchymal transition [J].
Kalluri, Raghu ;
Weinberg, Robert A. .
JOURNAL OF CLINICAL INVESTIGATION, 2009, 119 (06) :1420-1428
[13]   Interactions between JARID2 and Noncoding RNAs Regulate PRC2 Recruitment to Chromatin [J].
Kaneko, Syuzo ;
Bonasio, Roberto ;
Saldana-Meyer, Ricardo ;
Yoshida, Takahaki ;
Son, Jinsook ;
Nishino, Koichiro ;
Umezawa, Akihiro ;
Reinberg, Danny .
MOLECULAR CELL, 2014, 53 (02) :290-300
[14]   TGF-β1 induces human alveolar epithelial to mesenchymal cell transition (EMT) -: art. no. 56 [J].
Kasai, H ;
Allen, JT ;
Mason, RM ;
Kamimura, T ;
Zhang, Z .
RESPIRATORY RESEARCH, 2005, 6 (1)
[15]   Global profiling of histone and DNA methylation reveals epigenetic-based regulation of gene expression during epithelial to mesenchymal transition in prostate cells [J].
Ke, Xi-Song ;
Qu, Yi ;
Cheng, Yang ;
Li, Wen-Cheng ;
Rotter, Varda ;
Oyan, Anne Margrete ;
Kalland, Karl-Henning .
BMC GENOMICS, 2010, 11
[16]   The Organization of Histone H3 Modifications as Revealed by a Panel of Specific Monoclonal Antibodies [J].
Kimura, Hiroshi ;
Hayashi-Takanaka, Yoko ;
Goto, Yuji ;
Takizawa, Nanako ;
Nozaki, Naohito .
CELL STRUCTURE AND FUNCTION, 2008, 33 (01) :61-73
[17]   Molecular mechanisms and potential functions of histone demethylases [J].
Kooistra, Susanne Marije ;
Helin, Kristian .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2012, 13 (05) :297-311
[18]   Jarid2 and PRC2, partners in regulating gene expression [J].
Li, Gang ;
Margueron, Raphael ;
Ku, Manching ;
Chambon, Pierre ;
Bernstein, Bradley E. ;
Reinberg, Danny .
GENES & DEVELOPMENT, 2010, 24 (04) :368-380
[19]   Requirement of the histone demethylase LSD1 in Snail-mediated transcriptional repression during epithelial-mesenchymal transition [J].
Lin, T. ;
Ponn, A. ;
Hu, X. ;
Law, B. K. ;
Lu, J. .
ONCOGENE, 2010, 29 (35) :4896-4904
[20]   The Polycomb complex PRC2 and its mark in life [J].
Margueron, Raphael ;
Reinberg, Danny .
NATURE, 2011, 469 (7330) :343-349
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