Is cooperative oxygen binding by hemoglobin really understood?

被引:261
作者
Eaton, WA
Henry, ER
Hofrichter, J
Mozzarelli, A
机构
[1] NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA
[2] Univ Parma, Inst Biochem Sci, I-43100 Parma, Italy
[3] Univ Parma, Natl Inst Phys Matter, I-43100 Parma, Italy
关键词
D O I
10.1038/7586
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The enormous success of structural biology challenges the physical scientist. Can biophysical studies provide a truly deeper understanding of how a protein works than can be obtained from static structures and qualitative analysis of biochemical data? We address this question in a case study by presenting the key concepts and experimental results that have led to our current understanding of cooperative oxygen binding by hemoglobin, the paradigm of structure function relations in multisubunit proteins. We conclude that the underlying simplicity of the two-state allosteric mechanism could not have been demonstrated without novel physical experiments and a rigorous quantitative analysis.
引用
收藏
页码:351 / 358
页数:8
相关论文
共 45 条
[11]   Effects of NaCl on the linkages between O-2 binding and subunit assembly in human hemoglobin: Titration of the quaternary enhancement effect [J].
Doyle, ML ;
Holt, JM ;
Ackers, GK .
BIOPHYSICAL CHEMISTRY, 1997, 64 (1-3) :271-287
[12]  
Eaton W A, 1990, Adv Protein Chem, V40, P63, DOI 10.1016/S0065-3233(08)60287-9
[13]   APPLICATION OF LINEAR FREE-ENERGY RELATIONS TO PROTEIN CONFORMATIONAL-CHANGES - THE QUATERNARY STRUCTURAL-CHANGE OF HEMOGLOBIN [J].
EATON, WA ;
HENRY, ER ;
HOFRICHTER, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (10) :4472-4475
[14]   EXTENSIONS OF ALLOSTERIC MODEL FOR HAEMOGLOBIN [J].
EDELSTEIN, SJ .
NATURE, 1971, 230 (5291) :224-+
[15]  
EDSALL JT, 1980, FED PROC, V39, P226
[16]   THE ENERGY LANDSCAPES AND MOTIONS OF PROTEINS [J].
FRAUENFELDER, H ;
SLIGAR, SG ;
WOLYNES, PG .
SCIENCE, 1991, 254 (5038) :1598-1603
[17]   PHOTOCHEMICAL FORMATION OF A QUICKLY REACTING FORM OF HAEMOGLOBIN [J].
GIBSON, QH .
BIOCHEMICAL JOURNAL, 1959, 71 :293-303
[18]   COOPERATIVE FREE-ENERGIES FOR NESTED ALLOSTERIC MODELS AS APPLIED TO HUMAN-HEMOGLOBIN [J].
GILL, SJ ;
ROBERT, CH ;
COLETTA, M ;
DICERA, E ;
BRUNORI, M .
BIOPHYSICAL JOURNAL, 1986, 50 (04) :747-752
[19]   PROTEIN REACTION-KINETICS IN A ROOM-TEMPERATURE GLASS [J].
HAGEN, SJ ;
HOFRICHTER, J ;
EATON, WA .
SCIENCE, 1995, 269 (5226) :959-962
[20]   Can a two-state MWC allosteric model explain hemoglobin kinetics? [J].
Henry, ER ;
Jones, CM ;
Hofrichter, J ;
Eaton, WA .
BIOCHEMISTRY, 1997, 36 (21) :6511-6528