Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium

被引：199

作者：

Kurake, Naoyuki ^{[1
]}

Tanaka, Hiromasa ^{[1
]}

Ishikawa, Kenji ^{[1
]}

Kondo, Takashi ^{[3
]}

Sekine, Makoto ^{[1
]}

Nakamura, Kae ^{[2
]}

Kajiyama, Hiroaki ^{[2
]}

Kikkawa, Fumitaka ^{[2
]}

Mizuno, Masaaki ^{[2
]}

Hori, Masaru ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4848603, Japan

[2] Nagoya Univ, Grad Sch Med, Showa Ku, 65 Tsurumai Cho, Nagoya, Aichi 4668560, Japan

[3] Toyama Univ, 2630 Sugitani, Toyama 9300194, Japan

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2016年 / 605卷

关键词：

Apoptosis; Glioblastoma; Hydrogen peroxide; Nitrites; Plasma activated medium; Non-equilibrium atmospheric pressure plasma; ATMOSPHERIC-PRESSURE PLASMA; OXIDATIVE STRESS; INACTIVATION; DISCHARGE; ROS;

D O I：

10.1016/j.abb.2016.01.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-equilibrium atmospheric pressure plasmas generate a high electron density (on the order of 10(16) electrons per cm(-3)) using Ar gas. Culture medium in air at room temperature was plasma-irradiated for several hundred seconds. Tens of micromolar hydrogen peroxide (H2O2) and millimolar levels of nitrous ion (NO2- were detected in the plasma-irradiated culture medium (plasma activated medium; PAM) and selectively induced the apoptotic death of glioblastoma tumor cells, but did not kill normal mammary epithelial cells. A similar antitumor effect was induced by spiking the medium with comparable concentrations of H2O2 and NO2-. The PAM remained still a somewhat difference that it should also be assessed for understanding other latent mechanisms. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：102 / 108

页数：7

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