Ningmitai capsules have anti-inflammatory and pain-relieving effects in the chronic prostatitis/chronic pelvic pain syndrome mouse model through systemic immunity

被引:7
作者
Liu, Hanchao [1 ]
Wang, Zhenqing [1 ]
Xie, Qigen [1 ,2 ]
Chi, Ani [1 ]
Li, Yanqing [3 ]
Dai, Jian [1 ]
Zhang, Min [1 ]
Deng, Chunhua [1 ]
Liu, Guihua [3 ]
机构
[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Androl, Guangzhou, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Pediat Surg, Guangzhou, Peoples R China
[3] Sun Yat Sen Univ, Affiliated Hosp 6, Reprod Med Res Ctr, Guangzhou, Peoples R China
关键词
Ningmitai capsules; chronic prostatitis; chronic pelvic pain syndrome; anti-inflammation; pain-relieving; systemic immunity; MONOCYTE CHEMOATTRACTANT PROTEIN-1; MCP-1; DIFFERENTIATION; EXPRESSION; MEDIATORS; CELLS;
D O I
10.3389/fphar.2022.949316
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) seriously affects the physical and mental health of approximately 90% of males. Due to its complex and unclear etiology, the treatment methods that are currently available for chronic prostatitis/chronic pelvic pain syndrome are controversial, and their efficacy is unsatisfactory. At present, most researchers believe that this kind of prostatitis is caused by autoimmune inflammation. Chinese herbs, which are the essence of traditional Chinese medicine (TCM), are emerging treatment options for inflammation and immune diseases. In this experiment, we investigated the effect of Ningmitai capsules (a kind of traditional Chinese medicine widely used to treat lower urinary tract inflammation and pain in males) on chronic prostatitis/chronic pelvic pain syndrome in a non-obese diabetes-experimental autoimmune prostatitis (NOD-EAP) mouse model. First, by using bioinformatics analysis of data from the Encyclopedia of Traditional Chinese Medicine (ETCM) database, we found that quercetin, which is one of the main components of Ningmitai capsules, could reduce the secretion of CCL2 by inhibiting the MAPK pathway. In animal experiments, it was found that after Ningmitai treatment, the inflammation in mouse prostates was alleviated, the expression of CCL2, which is related to pain, and MAPK pathway components were downregulated, and the activation of the inflammatory NF-kappa B and STAT3 pathways was reduced. Pelvic pain and inflammation were relieved in mice with EAP. Due to the presence of the blood-prostate barrier, the drug may not completely reach the prostate directly and take effect locally. However, we found that after Ningmitai treatment, the proportions of proinflammatory CD11b(+)Ly6C(high) immune cells in the spleen, bloodstream (systemic immunity), and prostate (local immunity) were reduced. The infiltration of CD11b(+) immune cells into the spleen and prostate was decreased. These findings suggested that Ningmitai can treat chronic prostatitis/chronic pelvic pain syndrome by affecting systemic and local immunities through the CCL2-MAPK pathway.
引用
收藏
页数:14
相关论文
共 48 条
[1]   Chemokines and pain mechanisms [J].
Abbadie, Catherine ;
Bhangoo, Sonia ;
De Koninck, Yves ;
Malcangio, Marzia ;
Melik-Parsadaniantz, Stephane ;
White, Fletcher A. .
BRAIN RESEARCH REVIEWS, 2009, 60 (01) :125-134
[2]   Elevated levels of proinflammatory cytokines in the semen of patients with chronic prostatitis chronic pelvic pain syndrome [J].
Alexander, RB ;
Ponniah, S ;
Hasday, J ;
Hebel, JR .
UROLOGY, 1998, 52 (05) :744-749
[3]   Expression of IL-1β in supraspinal brain regions in rats with neuropathic pain [J].
Apkarian, A. Vania ;
Lavarello, Simona ;
Randolf, Anke ;
Berra, Hector H. ;
Chialvo, Dante R. ;
Besedovsky, Hugo O. ;
del Rey, Adriana .
NEUROSCIENCE LETTERS, 2006, 407 (02) :176-181
[4]  
Cai Hong-Cai, 2017, Zhonghua Nan Ke Xue, V23, P933
[5]   QUANTITATIVE ASSESSMENT OF TACTILE ALLODYNIA IN THE RAT PAW [J].
CHAPLAN, SR ;
BACH, FW ;
POGREL, JW ;
CHUNG, JM ;
YAKSH, TL .
JOURNAL OF NEUROSCIENCE METHODS, 1994, 53 (01) :55-63
[6]   The Anti-Inflammatory and Antioxidative Effects of Ningmitai Capsule in the Experimental Autoimmune Prostatitis Rat Model [J].
Chen, Haicheng ;
Xie, Yun ;
Deng, Cuncan ;
Zhang, Chi ;
Lv, Linyan ;
Yao, Jiahui ;
Sun, Xiangzhou ;
Deng, Chunhua ;
Liu, Guihua .
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2020, 2020
[7]   Chronic Prostatitis and Pelvic Pain Syndrome: Another Autoimmune Disease? [J].
Chen, Lei ;
Zhang, Meng ;
Liang, Chaozhao .
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS, 2021, 69 (01)
[8]   Prevalence and correlates of prostatitis in the health professionals follow-up study cohort [J].
Collins, MM ;
Meigs, JB ;
Barry, MJ ;
Corkery, EW ;
Giovannucci, E ;
Kawachi, I .
JOURNAL OF UROLOGY, 2002, 167 (03) :1363-1366
[9]   Recent advances of traditional Chinese medicine on the prevention and treatment of COVID-19 [J].
Dai Yu-Jie ;
Wan Shi-Yao ;
Gong Shuai-Shuai ;
Liu Jin-Cheng ;
Li Fang ;
Kou Jun-Ping .
CHINESE JOURNAL OF NATURAL MEDICINES, 2020, 18 (12) :881-889
[10]   Monocyte Chemoattractant Protein-1 (MCP-1): An Overview [J].
Deshmane, Satish L. ;
Kremlev, Sergey ;
Amini, Shohreh ;
Sawaya, Bassel E. .
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2009, 29 (06) :313-326