5′-poly(A) sequence as an effective leader for translation in eukaryotic cell-free systems

被引：17

作者：

Gudkov, AT ^{[1
]}

Ozerova, MV ^{[1
]}

Shiryaev, VM ^{[1
]}

Spirin, AS ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2005年 / 91卷 / 04期

关键词：

protein biosynthesis; poly(A) leader in mRNA; cell-free translation systems; translational enhancers; continuous-exchange cell-free (CECF) systems;

D O I：

10.1002/bit.20525

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Poly(A) sequence of 25 adenylic residues placed immediately before the start codons of the green fluorescent protein (GFP) and firefly luciferase (Luc) mRNAs is shown to provide a high rate of translation of the heterologous messages in eukaryotic cell-free translation systems. Also the poly(A) leader is found to provide the abolition of the inhibition of translation at excess mRNA concentrations. The possibility of the practical use of the constructs with the poly(A) leader for preparative protein production is demonstrated in the wheat germ continuous-exchange cell-free (CECF) translation system. (c) 2005 Wiley Periodicals, Inc.

引用

页码：468 / 473

页数：6

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