In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis

被引：58

作者：

Arun, Jeganathan ^{[1
]}

Selvakumar, Sundararaj ^{[1
]}

Sathishkumar, Ramamoorthy ^{[2
]}

Moovendhan, Meivelu ^{[2
]}

Ananthan, Gnanakkan ^{[2
]}

Maruthiah, Thirumalai ^{[3
]}

Palavesam, Arunachalam ^{[3
]}

机构：

[1] Madurai Kamaraj Univ, Sch Energy Environm & Nat Resources, Dept Nat Resources & Waste Recycling, Madurai 625021, Tamil Nadu, India

[2] Annamalai Univ, Fac Marine Sci, CAS Marine Biol, Parangipettai 608502, Tamil Nadu, India

[3] Manonmaniam Sundaranar Univ, Dept Anim Sci, Tirunelveli 627012, Tamil Nadu, India

来源：

CARBOHYDRATE POLYMERS | 2017年 / 155卷

关键词：

Exopolysaccharide; Antioxidant activity; Halolactibacillus miurensis; LIFE-STYLE FACTORS; OXIDATIVE STRESS; CAPACITY;

D O I：

10.1016/j.carbpol.2016.08.085

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography. EPS extracted from the supernatant yielded a 56.1% total carbohydrate content. The ash and moisture content were 12.1% and 1.5% respectively. Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis. FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region. In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately. HMEPS showed higher reducing ability against superoxide radical and potency in chelating the ferrous ions. 10 mg/ml of HMEPS was found equivalent to 2.7 units of ascorbic acid through the total antioxidant assay. Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：400 / 406

页数：7

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