Complex formation and functional versatility of Mre11 of budding yeast in recombination

被引：302
作者：
Usui, T
Ohta, T [1 ]
Oshiumi, H
Tomizawa, J
Ogawa, H
Ogawa, T
机构：
[1] Natl Inst Genet, Shizuoka 4118540, Japan
[2] Osaka Univ, Grad Sch Sci, Dept Biol, Osaka 5600043, Japan
来源：
CELL | 1998年 / 95卷 / 05期
基金：
日本学术振兴会;
关键词：
D O I：
10.1016/S0092-8674(00)81640-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Meiotic recombination of S. cerevisiae contains two temporally coupled processes, formation and processing of double-strand breaks (DSBs). Mre11 forms a complex with Rad50 and Xrs2, acting as the binding core, and participates in DSB processing. Although these proteins are also involved in DSB formation, Mre11 is not necessarily holding them. The C-terminal region of Mre11 is required only for DSB formation and binds to some meiotic proteins. The N-terminal half specifies nuclease activities that are collectively required for DSB processing. Mre11 has a DNA-binding site for DSB formation and another site for DSB processing. It has two regions to bind to Rad50. Mre11 repairs methyl methanesulfonate-induced DSBs by reactions that require the nuclease activities and those that do not.
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收藏
页码：705 / 716
页数：12
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